ArticleMolecular and cellular biology1999
Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 113 citations in OpenAlex.
- Stranded short nascent strand sequencing reveals the topology of DNA replication origins ineLife · 2026Article
- Replication initiation: Implications in genome integrity.DNA repair · 2021Review
- Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance.The Biochemical journal · 2020Article
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability.EMBO reports · 2019Article
- Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Chromosomal context and replication properties of ARS plasmids in Schizosaccharomyces pombe.Journal of biosciences · 2015Article
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Article
- Mechanisms for initiating cellular DNA replication.Annual review of biochemistry · 2013Review
- Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.BMC research notes · 2012Article
- Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.Genes & development · 2012Article
- Chromatin architectures at fission yeast transcriptional promoters and replication origins.Nucleic acids research · 2012Article
- New insights into replication origin characteristics in metazoans.Cell cycle (Georgetown, Tex.) · 2012Article
- Function of the INO80 chromatin remodeling complex in DNA replication.Frontiers in bioscience (Landmark edition) · 2012Review
- Kaposi's Sarcoma-Associated Herpesvirus Genome Replication, Partitioning, and Maintenance in Latency.Frontiers in microbiology · 2012Article
- Abundance of prereplicative complexes (Pre-RCs) facilitates recombinational repair under replication stress in fission yeast.The Journal of biological chemistry · 2011Article
- Eukaryotic DNA replication origins: many choices for appropriate answers.Nature reviews. Molecular cell biology · 2010Review
- Structural diversity and dynamics of genomic replication origins in Schizosaccharomyces pombe.The EMBO journal · 2010Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have determined functional elements required for autonomous replication of the Schizosaccharomyces pombe ars2004 that acts as an intrinsic chromosomal replication origin. Internal deletion analysis of a 940-bp fragment (ars2004M) showed three regions, I to III, to be required for autonomously replicating sequence (ARS) activity. Eight-base-pair substitutions in the 40-bp region I, composed of arrays of adenines on a DNA strand, resulted in a great reduction of ARS activity. Substitutions of region I with synthetic sequences showed that no specific sequence but rather repeats of three or more consecutive adenines or thymines, without interruption by guanine or cytosine, are required for the ARS activity. The 65-bp region III contains 11 repeats of the AAAAT sequence, while the 165-bp region II has short adenine or thymine stretches and a guanine- and cytosine-rich region which enhances ARS activity. All three regions in ars2004M can be replaced with 40-bp poly(dA/dT) fragments without reduction of ARS activity. Although spacer regions in the ars2004M enhance ARS activity, all could be deleted when an 40-bp poly(dA/dT) fragment was added in place of region I. Our results suggest that the origin activity of fission yeast replicators depends on the number of adenine/thymine stretches, the extent of their clustering, and presence of certain replication-enhancing elements.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.