ArticleMolecular and cellular biology1999
Roles of cell division and gene transcription in the methylation of CpG islands.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 135 citations in OpenAlex.
- Review
- Article
- SAM-DNMT3A, a strategy for induction of genome-wide DNA methylation, identifies DNA methylation as a vulnerability in ER-positive breast cancers.Nature communications · 2024Article
- Stress-induced epigenetic effects driven by maternal lactation in dairy cattle: a comethylation network approach.Epigenetics · 2024Article
- Environmental Factor Index (EFI): A Novel Approach to Measure the Strength of Environmental Influence on DNA Methylation in Identical Twins.Epigenomes · 2024Article
- Toward a comprehensive catalog of regulatory elements.Human genetics · 2023Review
- Characterisation of miRNA Expression in Dental Pulp Cells during Epigenetically-Driven Reparative Processes.International journal of molecular sciences · 2023Article
- DNA Methylation Profiling in a Cigarette Smoke-Exposed Mouse Model of Airway Inflammation.International journal of chronic obstructive pulmonary disease · 2022Article
- RAGE-mediated functional DNA methylated modification contributes to cigarette smoke-induced airway inflammation in mice.Bioscience reports · 2021Article
- Genome-wide DNA methylation alteration in prenatally stressed Brahman heifer calves with the advancement of age.Epigenetics · 2021Article
- Transcriptome Analysis of Dnmt3l Knock-Out Mice Derived Multipotent Mesenchymal Stem/Stromal Cells During Osteogenic Differentiation.Frontiers in cell and developmental biology · 2021Article
- Methylation Analysis in Monozygotic Twins With Treatment-Resistant Schizophrenia and Discordant Responses to Clozapine.Frontiers in psychiatry · 2021Article
- Article
- Zebularine-induced myeloma cell death is accompanied by decreased c-Myc expression.Cellular oncology (Dordrecht, Netherlands) · 2020Article
- Integrated Analysis of Tissue-Specific Promoter Methylation and Gene Expression Profile in Complex Diseases.International journal of molecular sciences · 2020Article
- Metabolism-associated genome-wide epigenetic changes in bovine oocytes during early lactation.Scientific reports · 2020Article
- The diverse roles of DNA methylation in mammalian development and disease.Nature reviews. Molecular cell biology · 2019Review
- Epigenetic Modifications in Acute Myeloid Leukemia: Prognosis, Treatment, and Heterogeneity.Frontiers in genetics · 2019Review
- Disruption of STAT3-DNMT1 interaction by SH-I-14 induces re-expression of tumor suppressor genes and inhibits growth of triple-negative breast tumor.Oncotarget · 2017Article
- Epigenome Aberrations: Emerging Driving Factors of the Clear Cell Renal Cell Carcinoma.International journal of molecular sciences · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
De novo methylation of CpG islands within the promoters of eukaryotic genes is often associated with their transcriptional repression, yet the methylation of CpG islands located downstream of promoters does not block transcription. We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second-exon CpG islands in T24 cells after 5-aza-2'-deoxycytidine (5-Aza-CdR) treatment to explore the relationship between CpG island methylation and gene transcription. The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter. We also examined the relationship between the remethylation of coding sequence CpG islands and gene transcription. The kinetics of remethylation of the p16 exon 2, PAX-6 exon 5, c-ABL exon 11, and MYF-3 exon 3 loci were examined following 5-Aza-CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells. Remethylation occurred most rapidly in the p16, PAX-6, and c-ABL genes, shown to be transcribed prior to drug treatment. These regions also exhibited higher levels of remethylation in single-cell clones and subclones derived from 5-Aza-CdR-treated T24 cells. Our data suggest that de novo methylation is not restricted to the S phase of the cell cycle and that transcription through CpG islands does not inhibit their remethylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.