ArticleMolecular and cellular biology1999
Mutations affecting a yeast mitochondrial inner membrane protein, pnt1p, block export of a mitochondrially synthesized fusion protein from the matrix.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Ribosome-Associated Mba1 Escorts Cox2 from Insertion Machinery to Maturing Assembly Intermediates.Molecular and cellular biology · 2016Article
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- Multiple roles of the Cox20 chaperone in assembly of Saccharomyces cerevisiae cytochrome c oxidase.Genetics · 2012Article
- Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase.The Journal of biological chemistry · 2011Article
- Article
- Roles of Oxa1-related inner-membrane translocases in assembly of respiratory chain complexes.Biochimica et biophysica acta · 2009Review
- Translocation of mitochondrially synthesized Cox2 domains from the matrix to the intermembrane space.Molecular and cellular biology · 2007Article
- The metalloprotease encoded by ATP23 has a dual function in processing and assembly of subunit 6 of mitochondrial ATPase.Molecular biology of the cell · 2007Article
- Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machinery.The Journal of cell biology · 2006Article
- Yeast Oxa1 interacts with mitochondrial ribosomes: the importance of the C-terminal region of Oxa1.The EMBO journal · 2003Article
- Article
- Interactions among COX1, COX2, and COX3 mRNA-specific translational activator proteins on the inner surface of the mitochondrial inner membrane of Saccharomyces cerevisiae.Molecular biology of the cell · 2003Article
- Resistance to pentamidine in Leishmania mexicana involves exclusion of the drug from the mitochondrion.Antimicrobial agents and chemotherapy · 2002Article
- Cox18p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner membrane.Molecular biology of the cell · 2002Article
- Role of positively charged transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Peripheral mitochondrial inner membrane protein, Mss2p, required for export of the mitochondrially coded Cox2p C tail in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Mba1, a novel component of the mitochondrial protein export machinery of the yeast Saccharomyces cerevisiae.The Journal of cell biology · 2001Article
- Oxa1p acts as a general membrane insertion machinery for proteins encoded by mitochondrial DNA.The EMBO journal · 2001Article
Corrections and comments
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2 authors at 1 institution in 1 country.
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Abstract
The machinery that inserts mitochondrially encoded proteins into the inner membrane and translocates their hydrophilic domains through the membrane is poorly understood. We have developed a genetic screen for Saccharomyces cerevisiae mutants defective in this export process. The screen is based on the fact that the hydrophilic polypeptide Arg8(m)p is exported from the matrix if it is synthesized within mitochondria as a bifunctional Cox2p-Arg8(m)p fusion protein. Since export of Arg8(m)p causes an Arg(-) phenotype, defective mutants can be selected as Arg(+). Here we show that mutations in the nuclear gene PNT1 block the translocation of mitochondrially encoded fusion proteins across the inner membrane. Pnt1p is a mitochondrial integral inner membrane protein that appears to have two hydrophilic domains in the matrix, flanking a central hydrophobic hairpin-like anchor. While an S. cerevisiae pnt1 deletion mutant was more sensitive to H(2)O(2) than the wild type was, it was respiration competent and able to export wild-type Cox2p. However, deletion of the PNT1 orthologue from Kluyveromyces lactis, KlPNT1, caused a clear nonrespiratory phenotype, absence of cytochrome oxidase activity, and a defect in the assembly of KlCox2p that appears to be due to a block of C-tail export. Since PNT1 was previously described as a gene affecting resistance to the antibiotic pentamidine, our data support a mitochondrial target for this drug.
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