ArticleMolecular and cellular biology1999
Rpn9 is required for efficient assembly of the yeast 26S proteasome.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Article
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- Long-Term Recreational Football Training and Health in Aging.International journal of environmental research and public health · 2020Review
- Lifelong Football Training: Effects on Autophagy and Healthy Longevity Promotion.Frontiers in physiology · 2019Article
- Expression and Regulation of Deubiquitinase-Resistant, Unanchored Ubiquitin Chains in Drosophila.Scientific reports · 2018Article
- Proteasomes tether to two distinct sites at the nuclear pore complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Temperature-sensitive retinoid isomerase activity of RPE65 mutants associated with Leber Congenital Amaurosis.Journal of biochemistry · 2015Article
- Solution structure of yeast Rpn9: insights into proteasome lid assembly.The Journal of biological chemistry · 2015Article
- Base-CP proteasome can serve as a platform for stepwise lid formation.Bioscience reports · 2015Article
- Rescue of enzymatic function for disease-associated RPE65 proteins containing various missense mutations in non-active sites.The Journal of biological chemistry · 2014Article
- Knockdown of APC/C-associated genes and its effect on viability and cell cycle of protozoan parasite of Trypanosoma brucei.Parasitology research · 2014Article
- The ubiquitin receptor S5a/Rpn10 links centrosomal proteasomes with dendrite development in the mammalian brain.Cell reports · 2013Article
- Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Dual function of Rpn5 in two PCI complexes, the 26S proteasome and COP9 signalosome.Molecular biology of the cell · 2011Article
- Structure characterization of the 26S proteasome.Biochimica et biophysica acta · 2011Review
- Down-regulation of the 26S proteasome subunit RPN9 inhibits viral systemic transport and alters plant vascular development.Plant physiology · 2006Article
- Participation of the proteasomal lid subunit Rpn11 in mitochondrial morphology and function is mapped to a distinct C-terminal domain.The Biochemical journal · 2004Article
- Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system.Molecular and cellular biology · 2003Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated the RPN9 gene by two-hybrid screening with, as bait, RPN10 (formerly SUN1), which encodes a multiubiquitin chain receptor residing in the regulatory particle of the 26S proteasome. Rpn9 is a nonessential subunit of the regulatory particle of the 26S proteasome, but the deletion of this gene results in temperature-sensitive growth. At the restrictive temperature, the Deltarpn9 strain accumulated multiubiquitinated proteins, indicating that the RPN9 function is needed for the 26S proteasome activity at a higher temperature. We analyzed the proteasome fractions separated by glycerol density gradient centrifugation by native polyacrylamide gel electrophoresis and found that a smaller amount of the 26S proteasome was produced in the Deltarpn9 cells and that the 26S proteasome was shifted to lighter fractions than expected. The incomplete proteasome complexes were found to accumulate in the Deltarpn9 cells. Furthermore, Rpn10 was not detected in the fractions containing proteasomes of the Deltarpn9 cells. These results indicate that Rpn9 is needed for incorporating Rpn10 into the 26S proteasome and that Rpn9 participates in the assembly and/or stability of the 26S proteasome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.