ArticleMolecular and cellular biology1999
AnCF, the CCAAT binding complex of Aspergillus nidulans, is essential for the formation of a DNase I-hypersensitive site in the 5' region of the amdS gene.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
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- Carbon Catabolite Repression in Filamentous Fungi.International journal of molecular sciences · 2017Review
- Microbial cellulases - Diversity & biotechnology with reference to mangrove environment: A review.Journal, genetic engineering & biotechnology · 2017Review
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- Bioprospecting thermophiles for cellulase production: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Review
- Pectate lyase affects pathogenicity in natural isolates of Colletotrichum coccodes and in pelA gene-disrupted and gene-overexpressing mutant lines.Molecular plant pathology · 2012Article
- Histone modifications and chromatin dynamics: a focus on filamentous fungi.FEMS microbiology reviews · 2008Review
- Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications.Mycopathologia · 2006Review
- Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina.Eukaryotic cell · 2006Article
- Nucleosome transactions on the Hypocrea jecorina (Trichoderma reesei) cellulase promoter cbh2 associated with cellulase induction.Molecular genetics and genomics : MGG · 2003Article
- Transcriptional regulation of xyn2 in Hypocrea jecorina.Eukaryotic cell · 2003Article
- Regulation of the acuF gene, encoding phosphoenolpyruvate carboxykinase in the filamentous fungus Aspergillus nidulans.Journal of bacteriology · 2002Article
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Abstract
The CCAAT sequence in the amdS promoter of Aspergillus nidulans is recognized by AnCF, a complex consisting of the three evolutionary conserved subunits HapB, HapC, and HapE. In this study we have investigated the effect of AnCF on the chromatin structure of the amdS gene. The AnCF complex and the CCAAT sequence were found to be necessary for the formation of a nucleosome-free, DNase I-hypersensitive region in the 5' region of the amdS gene. Deletion of the hapE gene results in loss of the DNase I-hypersensitive site, and the positioning of nucleosomes over the transcriptional start point is lost. Likewise, a point mutation in the CCAAT motif, as well as a 530-bp deletion which removes the CCAAT box, results in the loss of the DNase I-hypersensitive region. The DNase I-hypersensitive region and the nucleosome positioning can be restored by insertion of a 35-bp oligonucleotide carrying the CCAAT motif. A DNase I-hypersensitive region has been found in the CCAAT-containing fmdS gene and was also hapE dependent. These data indicate a critical role for the AnCF complex in establishing an open chromatin structure in A. nidulans.
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