ArticleThe Plant cell1999
Identification of two essential sequence elements in the nonconsensus type II PatpB-290 plastid promoter by using plastid transcription extracts from cultured tobacco BY-2 cells.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 71 citations in OpenAlex.
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Chimeric deletion mutation of rpoC2 underlies the leaf-patterning of Clivia miniata var. variegata.Plant cell reports · 2023Article
- The chloroplast redox-responsive transcriptome of solanaceous plants reveals significant nuclear gene regulatory motifs associated to stress acclimation.Plant molecular biology · 2022Article
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The plastid genome as a chassis for synthetic biology-enabled metabolic engineering: players in gene expression.Plant cell reports · 2018Review
- In vitro promoter recognition by the catalytic subunit of plant phage-type RNA polymerases.Plant molecular biology · 2016Article
- The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps.Journal of experimental botany · 2014Article
- Recent advances in the study of chloroplast gene expression and its evolution.Frontiers in plant science · 2014Review
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- The complete nucleotide sequences of the 5 genetically distinct plastid genomes of Oenothera, subsection Oenothera: II. A microevolutionary view using bioinformatics and formal genetic data.Molecular biology and evolution · 2008Article
- The complete nucleotide sequences of the five genetically distinct plastid genomes of Oenothera, subsection Oenothera: I. sequence evaluation and plastome evolution.Nucleic acids research · 2008Article
- Impaired function of the phage-type RNA polymerase RpoTp in transcription of chloroplast genes is compensated by a second phage-type RNA polymerase.Nucleic acids research · 2008Article
- High diversity of plastidial promoters in Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2007Article
- Arabidopsis phage-type RNA polymerases: accurate in vitro transcription of organellar genes.The Plant cell · 2007Article
- Functional classification, genomic organization, putatively cis-acting regulatory elements, and relationship to quantitative trait loci, of sorghum genes with rhizome-enriched expression.Plant physiology · 2006Article
- Sub-plastidial localization of two different phage-type RNA polymerases in spinach chloroplasts.Nucleic acids research · 2006Article
- Glutamyl-tRNA mediates a switch in RNA polymerase use during chloroplast biogenesis.EMBO reports · 2005Article
- Analysis of developing maize plastids reveals two mRNA stability classes correlating with RNA polymerase type.EMBO reports · 2004Article
- Overexpression of phage-type RNA polymerase RpoTp in tobacco demonstrates its role in chloroplast transcription by recognizing a distinct promoter type.Nucleic acids research · 2004Article
- MFP1 is a thylakoid-associated, nucleoid-binding protein with a coiled-coil structure.Nucleic acids research · 2003Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In higher plants, plastid genes are transcribed by at least two types of DNA-dependent RNA polymerases. One of them is the well-known plastid-encoded prokaryotic type of polymerase that recognizes sigma(70)-type promoters consisting of -35 and -10 consensus elements. The other recently recognized RNA polymerase has been found to be encoded entirely in the nucleus, and it recognizes a completely different set of promoters, designated previously as nonconsensus type II (NCII) promoters. Here, we report the development of an in vitro transcription system using nonphotosynthetic plastids of cultured tobacco BY-2 cells. This system preferentially and accurately initiates transcription from NCII promoters. The conditions for in vitro transcription were optimized by using the tobacco PatpB-290 promoter, which has been found to be the most highly expressed NCII promoter in vivo. Analysis of in vitro transcription initiation in a series of PatpB-290 5' deletion constructs revealed that sequences upstream of nucleotide -41 do not influence the transcriptional activity of this promoter. A 43-bp region (nucleotides -35 to +8) was further analyzed by introducing single or multiple nucleotide substitutions into two regions (box I and box II) of high sequence conservation. We report here that the ATAGAA sequence comprising box II and the -11 to +4 region (relative to transcription initiation) in box I significantly influence the activity of this NCII promoter.
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