ArticleJournal of clinical microbiology1999
Development of a species-specific PCR-restriction fragment length polymorphism analysis procedure for identification of Madurella mycetomatis.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 110 citations in OpenAlex.
- A qPCR identification scheme to differentiate actinomycetoma from eumycetoma.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Mycetoma.PLoS neglected tropical diseases · 2026Review
- A rare case ofIDCases · 2026Article
- Sandalwood Sesquiterpene (Molecules (Basel, Switzerland) · 2025Article
- Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker.PLoS neglected tropical diseases · 2024Article
- The combination of manogepix and itraconazole is synergistic and inhibits the growth of Madurella mycetomatis in vitro but not in vivo.Medical mycology · 2023Article
- Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent.PLoS neglected tropical diseases · 2023Article
- Diagnostics to support mycetoma management-Development of two target product profiles.Tropical medicine & international health : TM & IH · 2022Article
- Ultrasound-guided fine-needle aspiration cytology significantly improved mycetoma diagnosis.Journal of the European Academy of Dermatology and Venereology : JEADV · 2022Article
- Inhibiting DHN- and DOPA-melanin biosynthesis pathway increased the therapeutic value of itraconazole in Madurella mycetomatis infected Galleria mellonella.Medical mycology · 2022Article
- Comparison of Disc Diffusion, Etest, and a Modified CLSI Broth Microdilution Method forAntimicrobial agents and chemotherapy · 2021Article
- The synthetic synergistic cinnamon oil CIN-102 is active against Madurella mycetomatis, the most common causative agent of mycetoma.PLoS neglected tropical diseases · 2021Article
- The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach.PLoS neglected tropical diseases · 2020Article
- Diagnostic implications of mycetoma derived from Madurella pseudomycetomatis isolates from Mexico.Journal of the European Academy of Dermatology and Venereology : JEADV · 2020Article
- Madurella mycetomatis causing eumycetoma medical treatment: The challenges and prospects.PLoS neglected tropical diseases · 2020Review
- The genus Madurella: Molecular identification and epidemiology in Sudan.PLoS neglected tropical diseases · 2020Article
- Madurella mycetomatis, the main causative agent of eumycetoma, is highly susceptible to olorofim.The Journal of antimicrobial chemotherapy · 2020Article
- Mycetoma epidemiology, diagnosis management, and outcome in three hospital centres in Senegal from 2008 to 2018.PloS one · 2020Article
- Madurella real-time PCR, a novel approach for eumycetoma diagnosis.PLoS neglected tropical diseases · 2020Article
- The Accuracy of Histopathological and Cytopathological Techniques in the Identification of the Mycetoma Causative Agents.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Madurella mycetomatis is the commonest cause of eumycetoma in Sudan and other countries in tropical Africa. Currently, the early diagnosis of mycetoma is difficult. In attempting to improve the identification of M. mycetomatis and, consequently, the diagnosis of mycetoma, we have developed specific oligonucleotide primers based on the sequence of the internal transcribed spacer (ITS) regions spacing the genes encoding the fungal ribosomal RNAs. The ITS regions were amplified with universal primers and sequenced, and then two sets of species-specific primers were designed which specifically amplify parts of the ITS and the 5.8S ribosomal DNA gene. The new primers were tested for specificity with DNA isolated from human mycetoma lesions and DNA extracted from cultures of M. mycetomatis reference strains and related fungi as well as human DNA. To study the genetic variability of the ITS regions of M. mycetomatis, ITS amplicons were obtained from 25 different clinical isolates and subjected to restriction fragment length polymorphism (RFLP) analysis with CfoI, HaeIII, MspI, Sau3AI, RsaI, and SpeI restriction enzymes. RFLP analysis of the ITS region did not reveal even a single difference, indicating the homogeneity of the isolates analyzed during the current study.
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