ArticleJournal of clinical microbiology1999
Molecular analysis of riboflavin synthesis genes in Bartonella henselae and use of the ribC gene for differentiation of Bartonella species by PCR.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- Opportunities for Riboswitch Inhibition by Targeting Co-Transcriptional RNA Folding Events.International journal of molecular sciences · 2024Review
- Under-diagnosis of vector-borne diseases among individuals suspected of having Scrub Typhus in South Korea.PloS one · 2023Article
- Riboflavin, a Potent Neuroprotective Vitamin: Focus on Flavivirus and Alphavirus Proteases.Microorganisms · 2022Article
- Genomic Analysis ofFrontiers in microbiology · 2021Article
- Carrion's Disease: the Sound of Silence.Clinical microbiology reviews · 2018Review
- Babesia spp. and other pathogens in ticks recovered from domestic dogs in Denmark.Parasites & vectors · 2015Article
- Oroya fever and verruga peruana: bartonelloses unique to South America.PLoS neglected tropical diseases · 2014Review
- Strategy for identification & characterization of Bartonella henselae with conventional & molecular methods.The Indian journal of medical research · 2013Article
- Molecular and functional characterization of riboflavin specific transport system in rat brain capillary endothelial cells.Brain research · 2012Article
- Granulomatous hepatitis due to Bartonella henselae infection in an immunocompetent patient.BMC infectious diseases · 2012Article
- Molecular detection of Rickettsia felis, Bartonella henselae, and B. clarridgeiae in fleas from domestic dogs and cats in Malaysia.The American journal of tropical medicine and hygiene · 2011Article
- Multi-locus sequence analysis reveals profound genetic diversity among isolates of the human pathogen Bartonella bacilliformis.PLoS neglected tropical diseases · 2011Article
- Evolutional and geographical relationships of Bartonella grahamii isolates from wild rodents by multi-locus sequencing analysis.Microbial ecology · 2009Article
- Molecular evidence of Bartonella DNA in ixodid ticks in Czechia.Folia microbiologica · 2007Article
- Potential limitations of the 16S-23S rRNA intergenic region for molecular detection of Bartonella species.Journal of clinical microbiology · 2005Article
- Molecular Methods in the Diagnosis of Endocarditis.Current infectious disease reports · 2004Article
- Characterization of the natural population of Bartonella henselae by multilocus sequence typing.Journal of clinical microbiology · 2003Article
- Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC).Journal of clinical microbiology · 2003Article
- Bartonella henselae in Ixodes ricinus ticks (Acari: Ixodida) removed from humans, Belluno province, Italy.Emerging infectious diseases · 2003Article
- Diagnosis of Bartonella endocarditis by a real-time nested PCR assay using serum.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biosynthesis pathway for riboflavin (vitamin B(2)), the precursor of the essential cofactors flavin mononucleotide and flavin adenine dinucleotide, is present in bacteria and plants but is absent in vertebrates. Due to their conservation in bacterial species and their absence in humans, the riboflavin synthesis genes should be well suited either for detection of bacterial DNA in human specimens or for the differentiation of pathogenic bacteria by molecular techniques. A DNA fragment carrying the genes ribD, ribC, and ribE, which encode homologues of riboflavin deaminase (RibD) and subunits of riboflavin synthetase (RibC and RibE), respectively, was isolated from a plasmid-based DNA library of the human pathogen Bartonella henselae by complementation of a ribC mutation in Escherichia coli. Sequence analysis of the ribC gene region in strains of B. henselae, which were previously shown to be genetically different, revealed that the ribC gene is highly conserved at the species level. PCR amplification with primers derived from the ribC locus of B. henselae was used to isolate the corresponding DNA regions in B. bacilliformis, B. clarridgeiae, and B. quintana. Sequence analysis indicated that the riboflavin synthesis genes are conserved and show the same operon-like genetic organization in all four Bartonella species. Primer oligonucleotides designed on the basis of localized differences within the ribC DNA region were successfully used to develop species-specific PCR assays for the differentiation of B. henselae, B. clarridgeiae, B. quintana, and B. bacilliformis. The results obtained indicate that the riboflavin synthesis genes are excellent targets for PCR-directed differentiation of these emerging pathogens. The PCR assays developed should increase our diagnostic potential to differentiate Bartonella species, especially B. henselae and the newly recognized species B. clarridgeiae.
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