ArticleThe American journal of pathology1999
Cloning and expression of the rat nephrin homolog.
Article in The American journal of pathology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 72 citations in OpenAlex.
- Loss of S1P Lyase Expression in Human Podocytes Causes a Reduction in Nephrin Expression That Involves PKCδ Activation.International journal of molecular sciences · 2023Article
- Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules.World journal of nephrology · 2014Review
- Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment.PloS one · 2012Article
- Slit diaphragm dysfunction in proteinuric states: identification of novel therapeutic targets for nephrotic syndrome.Clinical and experimental nephrology · 2009Review
- Inducible overexpression of sFlt-1 in podocytes ameliorates glomerulopathy in diabetic mice.Diabetes · 2008Article
- Angiotensin II type 1 and type 2 receptors play opposite roles in regulating the barrier function of kidney glomerular capillary wall.The American journal of pathology · 2007Article
- Nephrin forms a complex with adherens junction proteins and CASK in podocytes and in Madin-Darby canine kidney cells expressing nephrin.The American journal of pathology · 2004Article
- Involvement of lipid rafts in nephrin phosphorylation and organization of the glomerular slit diaphragm.The American journal of pathology · 2001Article
- Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney.The American journal of pathology · 1999Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite of the increased availability of genetically modified mouse strains, the experimental models in the rat have provided the most widely employed and versatile models for the study of renal pathophysiology and functional genetics. The identification of the human gene mutated in the congenital nephrotic syndrome of the Finnish type (NPHS1) has recently been reported, and its protein product has been termed nephrin. Here we report the molecular cloning and characterization of rat nephrin cDNA. Rat nephrin cDNA has an open reading frame of 3705 bp, shows 82% sequence identity with human nephrin cDNA, and shows characteristic rat-specific splicing variants. The translated nucleotide sequence has 89% sequence identity at the amino acid level. The signal sequence, glycosylation, and cysteine localization patterns are nearly identical to those of human nephrin. As in the human, the rat nephrin transcript is expressed in a tissue-restricted pattern. Antipeptide antibodies raised to the intracellular nephrin-specific domain identified immunoreactivity exclusively within the rat kidney glomerulus by indirect immunofluorescence. Initial results with semiquantitative reverse transcriptase-polymerase chain reaction analysis showed a remarkable down-regulation of nephrin-specific mRNA in the puromycin nephrosis of the rat.
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