ArticlePlant molecular biology1999
Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages.SpringerPlus · 2013Article
- Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis.BMC plant biology · 2006Article
- Isolation, sequence analysis, and expression studies of florally expressed cDNAs in Arabidopsis.Plant molecular biology · 2003Article
- The endosperm and the embryo of Arabidopsis thaliana are independently transformed through infiltration by Agrobacterium tumefaciens.Transgenic research · 2003Article
- The Arabidopsis SKP1-like genes present a spectrum of expression profiles.Plant molecular biology · 2003Article
- Temporal and tissue-specific expression of the tobacco ntf4 MAP kinase.Plant molecular biology · 2001Article
- The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana.Genetics · 2000Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analysis of one of them, named BnM3.4, by in situ hybridization, showed that it is expressed specifically and at a high level in the rapeseed microspore. The specificity in its profile of expression is most likely transcriptionally controlled as a similar pattern of expression was also observed in Arabidopsis thaliana plants transformed by the BnM3.4 promoter fused to the reporter GUS-coding sequence. The putative BnM3.4 promoter contains three dispersed copies of a motif described previously in the promoters of several genes expressed in the male gametophyte. The BnM3.4 gene encodes a predicted novel proline-rich protein of 23.4 kDa which may interact with cytoskeletal components or have a structural role in the cell wall.
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