ArticleProceedings of the National Academy of Sciences of the United States of America1999
Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogen Pseudomonas syringae pathovar phaseolicola.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 313 citations in OpenAlex.
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- Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry.Molecular plant pathology · 2024Article
- Suppression of NLR-mediated plant immune detection by bacterial pathogens.Journal of experimental botany · 2023Review
- Cooperative virulence via the collective action of secreted pathogen effectors.Nature microbiology · 2023Article
- Metaeffector interactions modulate the type III effector-triggered immunity load of Pseudomonas syringae.PLoS pathogens · 2022Article
- Molecular Characteristics and Gonococcal Genetic Island Carrying Status of Thirty-SevenInfection and drug resistance · 2022Article
- Signaling Pathways and Downstream Effectors of Host Innate Immunity in Plants.International journal of molecular sciences · 2021Review
- ThePlant communications · 2021Article
- A Large Tn7-like Transposon Confers Hyper-Resistance to Copper inApplied and environmental microbiology · 2021Article
- Diversity within species: interpreting strains in microbiomes.Nature reviews. Microbiology · 2020Review
- Cherry picking by pseudomonads: After a century of research on canker, genomics provides insights into the evolution of pathogenicity towards stone fruits.Plant pathology · 2020Review
- Article
- Plasmid composition and the chpG gene determine the virulence level of Clavibacter capsici natural isolates in pepper.Molecular plant pathology · 2020Article
- GenesFrontiers in plant science · 2020Article
- BacTag - a pipeline for fast and accurate gene and allele typing in bacterial sequencing data based on database preprocessing.BMC genomics · 2019Article
- The Role of Proteases in the Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2019Review
- Review
- The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes ofMobile DNA · 2019Article
- Coping with Environmental Eukaryotes; Identification ofMicroorganisms · 2018Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 154-kb plasmid was cured from race 7 strain 1449B of the phytopathogen Pseudomonas syringae pv. phaseolicola (Pph). Cured strains lost virulence toward bean, causing the hypersensitive reaction in previously susceptible cultivars. Restoration of virulence was achieved by complementation with cosmid clones spanning a 30-kb region of the plasmid that contained previously identified avirulence (avr) genes avrD, avrPphC, and avrPphF. Single transposon insertions at multiple sites (including one located in avrPphF) abolished restoration of virulence by genomic clones. Sequencing 11 kb of the complementing region identified three potential virulence (vir) genes that were predicted to encode hydrophilic proteins and shared the hrp-box promoter motif indicating regulation by HrpL. One gene achieved partial restoration of virulence when cloned on its own and therefore was designated virPphA as the first (A) gene from Pph to be identified for virulence function. In soybean, virPphA acted as an avr gene controlling expression of a rapid cultivar-specific hypersensitive reaction. Sequencing also revealed the presence of homologs of the insertion sequence IS100 from Yersinia and transposase Tn501 from P. aeruginosa. The proximity of several avr and vir genes together with mobile elements, as well as G+C content significantly lower than that expected for P. syringae, indicates that we have located a plasmid-borne pathogenicity island equivalent to those found in mammalian pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.