ArticleProceedings of the National Academy of Sciences of the United States of America1999
Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 116 citations in OpenAlex.
- Native Top-Down Analysis of Membrane Protein Complexes Directly From In Vitro and Native Membranes.Molecular & cellular proteomics : MCP · 2025Article
- Kinetic isotope effect reveals rate-limiting step in green-to-red photoconvertible fluorescent proteins.Protein science : a publication of the Protein Society · 2024Article
- Emulation of the structure of the Saposin protein fold by a lung surfactant peptide construct of surfactant Protein B.PloS one · 2022Article
- Targeting a Subset of the Membrane Proteome for Top-Down Mass Spectrometry: Introducing the Proteolipidome.Proteomes · 2020Article
- Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.Nature methods · 2019Review
- Rapid LC-MS Method for Accurate Molecular Weight Determination of Membrane and Hydrophobic Proteins.Analytical chemistry · 2018Article
- Mapping the energy landscape for second-stage folding of a single membrane protein.Nature chemical biology · 2015Article
- New mass-spectrometry-compatible degradable surfactant for tissue proteomics.Journal of proteome research · 2015Article
- Review
- Integral membrane proteins and bilayer proteomics.Analytical chemistry · 2013Review
- A designed inhibitor of a CLC antiporter blocks function through a unique binding mode.Chemistry & biology · 2012Article
- Profiling of integral membrane proteins and their post translational modifications using high-resolution mass spectrometry.Methods (San Diego, Calif.) · 2011Review
- Review
- Hydrophobic Proteome Analysis of Triple Negative and Hormone-Receptor-Positive-Her2-Negative Breast Cancer by Mass Spectrometer.Clinical proteomics · 2010Article
- Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociation.Molecular & cellular proteomics : MCP · 2010Article
- A thiolate anion buried within the hydrocarbon ruler perturbs PagP lipid acyl chain selection.Biochemistry · 2010Article
- Detection of two distinct forms of apoC-I in great apes.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2010Article
- Hydrophobic Fractionation Enhances Novel Protein Detection by Mass Spectrometry in Triple Negative Breast Cancer.Journal of proteomics & bioinformatics · 2010Article
- Structural studies of the Sputnik virophage.Journal of virology · 2010Article
- Mass spectral analyses of the two major apolipoproteins of great ape high density lipoproteins.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Genes encoding membrane proteins comprise a substantial proportion of genomes sequenced to date, but ability to perform structural studies on this portion of the proteome is limited. Electrospray ionization-MS (ESI-MS) of an intact protein generates a profile defining the native covalent state of the gene product and its heterogeneity. Here we apply ESI-MS technology with accuracy exceeding 0.01% to a hydrophobic membrane protein with 12-transmembrane alpha-helices, the full-length lactose permease from Escherichia coli. Furthermore, ESI-MS is used to titrate reactive thiols with N-ethylmaleimide. Treatment of the native protein solubilized in detergent micelles reveals only two reactive thiols, and both are protected by a substrate analog.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.