ArticleProceedings of the National Academy of Sciences of the United States of America1999
Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Review
- A Missense Genetic Variant in LRRC16A/CARMIL1 Improves Acute Respiratory Distress Syndrome Survival by Attenuating Platelet Count Decline.American journal of respiratory and critical care medicine · 2017Article
- Article
- Homologous SV40 RNA trans-splicing: a new mechanism for diversification of viral sequences and phenotypes.RNA biology · 2013Article
- Applying the brakes to multisite SR protein phosphorylation: substrate-induced effects on the splicing kinase SRPK1.Biochemistry · 2011Article
- Phosphorylation mechanism and structure of serine-arginine protein kinases.The FEBS journal · 2011Review
- Mechanism of dephosphorylation of the SR protein ASF/SF2 by protein phosphatase 1.Journal of molecular biology · 2010Article
- Cooperative-binding and splicing-repressive properties of hnRNP A1.Molecular and cellular biology · 2009Article
- Differential 3' splice site recognition of SMN1 and SMN2 transcripts by U2AF and U2 snRNP.RNA (New York, N.Y.) · 2009Article
- The SR protein family.Genome biology · 2009Review
- Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C.Cellular & molecular biology letters · 2009Article
- Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing.PloS one · 2007Article
- Sing the genome electric: excited cells adjust their splicing.PLoS biology · 2007Article
- The splicing regulators Tra and Tra2 are unusually potent activators of pre-mRNA splicing.Nucleic acids research · 2006Article
- Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation.Molecular and cellular biology · 2003Article
- Ischemia induces a translocation of the splicing factor tra2-beta 1 and changes alternative splicing patterns in the brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.RNA (New York, N.Y.) · 2001Article
- The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.The EMBO journal · 2001Article
- Localization of an exonic splicing enhancer responsible for mammalian natural trans-splicing.Nucleic acids research · 2001Article
- Identification of single nucleotide polymorphisms (SNPs) and other sequence changes and estimation of nucleotide diversity in coding and flanking regions of the NMDAR1 receptor gene in schizophrenic patients.Molecular psychiatry · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Exonic splicing enhancers (ESEs) activate pre-mRNA splicing by promoting the use of the flanking splice sites. They are recognized by members of the serine/arginine-rich (SR) family of proteins, such as splicing factor 2/alternative splicing factor (SF2/ASF), which recruit basal splicing factors to form the initial complexes during spliceosome assembly. The in vitro splicing kinetics of an ESE-dependent IgM pre-mRNA suggested that an SF2/ASF-specific ESE has additional functions later in the splicing reaction, after the completion of the first catalytic step. A bimolecular exon ligation assay, which physically uncouples the first and second catalytic steps of splicing in a trans-splicing reaction, was adapted to test the function of the ESE after the first step. A 3' exon containing the SF2/ASF-specific ESE underwent bimolecular exon ligation, whereas 3' exons without the ESE or with control sequences did not. The ESE-dependent trans-splicing reaction occurred after inactivation of U1 or U2 small nuclear ribonucleoprotein particles, compatible with a functional assay for events after the first step of splicing. The ESE-dependent step appears to take place before the ATP-independent part of the second catalytic step. Bimolecular exon ligation also occurred in an S100 cytosolic extract, requiring both the SF2/ASF-dependent ESE and complementation with SF2/ASF. These data suggest that some ESEs can act late in the splicing reaction, together with appropriate SR proteins, to enhance the second catalytic step of splicing.
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