ArticleProceedings of the National Academy of Sciences of the United States of America1999
Padlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 58 citations in OpenAlex.
- Sequence-Specific Targeting of GC-Rich Gene Loci by Parallel Triplex-Forming Oligonucleotides Containing a Modified Nucleobase.bioRxiv : the preprint server for biology · 2026Article
- Fluorous-Directed Clamping Stabilizes Triple-Helical DNA.ACS omega · 2026Article
- Structural optimization of pseudorotaxane-forming oligonucleotides for efficient and stable complex formation.Nucleic acids research · 2018Article
- A capture approach for supercoiled plasmid DNA using a triplex-forming oligonucleotide.Nucleic acids research · 2013Article
- Using triplex-forming oligonucleotide probes for the reagentless, electrochemical detection of double-stranded DNA.Analytical chemistry · 2010Article
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Coupling across a DNA helical turn yields a hybrid DNA/organic catenane doubly tailed with functional termini.Journal of the American Chemical Society · 2008Article
- Covalent ligation studies on the human telomere quadruplex.Nucleic acids research · 2005Article
- Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level.Nucleic acids research · 2003Article
- Padlock oligonucleotides as a tool for labeling superhelical DNA.Nucleic acids research · 2002Article
- Rolling-circle amplification under topological constraints.Nucleic acids research · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An oligonucleotide was circularized around double-stranded DNA thanks to triple helix formation. Short oligonucleotides are known to be able to form DNA triple helices by binding into the DNA major groove at an oligopurine.oligopyrimidine sequence. After sequence-specific recognition of a double-stranded DNA target through triple helix formation, the ends of the triplex-forming oligonucleotide were joined through the action of T4 DNA ligase, thus creating a circular DNA molecule catenated to the plasmid containing the target sequence. The labeling of the double-stranded DNA sequence has been carried out without any chemical or enzymatic modification of this sequence. These "padlock" oligonucleotides provide a tool to attach a noncovalent tag in an irreversible way to supercoiled plasmid or other double-stranded DNAs. Such a complex may find applications in the development of new techniques for duplex DNA detection or plasmid delivery methods for gene therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.