ArticleGenes & development1999
Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 94 citations in OpenAlex.
- Phosphate-dependent nuclear export via a non-classical NES class recognized by exportin Msn5.Nature communications · 2025Article
- Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine.The Journal of cell biology · 2019Article
- Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.Cell cycle (Georgetown, Tex.) · 2019Article
- Spatial and temporal signal processing and decision making by MAPK pathways.The Journal of cell biology · 2017Review
- Barcode Sequencing Screen Identifies SUB1 as a Regulator of Yeast Pheromone Inducible Genes.G3 (Bethesda, Md.) · 2016Article
- Role of Polarized G Protein Signaling in Tracking Pheromone Gradients.Developmental cell · 2015Article
- Pheromone-induced morphogenesis and gradient tracking are dependent on the MAPK Fus3 binding to Gα.Molecular biology of the cell · 2015Article
- Compartmentalization of a bistable switch enables memory to cross a feedback-driven transition.Cell · 2015Article
- Genome-wide investigation of the role of the tRNA nuclear-cytoplasmic trafficking pathway in regulation of the yeast Saccharomyces cerevisiae transcriptome and proteome.Molecular and cellular biology · 2013Article
- A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.Biochimica et biophysica acta · 2013Article
- Molecular basis of the functional distinction between Cln1 and Cln2 cyclins.Cell cycle (Georgetown, Tex.) · 2012Article
- The karyopherin Kap95 and the C-termini of Rfa1, Rfa2, and Rfa3 are necessary for efficient nuclear import of functional RPA complex proteins in Saccharomyces cerevisiae.DNA and cell biology · 2011Article
- Dynamic localization of Fus3 mitogen-activated protein kinase is necessary to evoke appropriate responses and avoid cytotoxic effects.Molecular and cellular biology · 2010Article
- Common genetic pathways regulate organ-specific infection-related development in the rice blast fungus.The Plant cell · 2010Article
- Chemical gradients and chemotropism in yeast.Cold Spring Harbor perspectives in biology · 2009Review
- Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.The EMBO journal · 2007Article
- Mechanism underlying the iron-dependent nuclear export of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae.Molecular biology of the cell · 2007Article
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
- Classical nuclear localization signals: definition, function, and interaction with importin alpha.The Journal of biological chemistry · 2007Review
- Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast.Molecular and cellular biology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Far1p is a bifunctional protein that is required to arrest the cell cycle and to establish cell polarity during yeast mating. Far1p is localized predominantly in the nucleus but accumulates in the cytoplasm in cells exposed to pheromones. Here we show that Far1p functions in both subcellular compartments: nuclear Far1p is required to arrest the cell cycle, whereas cytoplasmic Far1p is involved in the establishment of cell polarity. The subcellular localization of Far1p is regulated by two mechanisms: (1) Far1p contains a functional bipartite nuclear localization signal (NLS), and (2) Far1p is exported from the nucleus by Msn5p/Ste21p, a member of the exportin family. Cells deleted for Msn5p/Ste21p failed to export Far1p in response to pheromones, whereas overexpression of Msn5p/Ste21p was sufficient to accumulate Far1p in the cytoplasm in the absence of pheromones. Msn5p/Ste21p was localized in the nucleus and interacted with Far1p in a manner dependent on GTP-bound Gsp1p. Two-hybrid analysis identified a small fragment within Far1p that is necessary and sufficient for binding to Msn5p/Ste21p, and is also required to export Far1p in vivo. Finally, similar to Deltamsn5/ste21 strains, cells expressing a mutant Far1p, which can no longer be exported, exhibit a mating defect, but are able to arrest their cell cycle in response to pheromones. Taken together, our results suggest that nuclear export of Far1p by Msn5p/Ste21p coordinates the two separable functions of Far1p during mating.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.