ArticleGenes & development1999
Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
82 citing papers in PubMed, 201 citations in OpenAlex.
- Centrosome-Signaling Pathway Crosstalk: A Core Hub From Cellular Homeostasis to Disease.Cytoskeleton (Hoboken, N.J.) · 2026Review
- The Impact of Centrosome Pathologies on Ovarian Cancer Development and Progression with a Focus on Centrosomes as Therapeutic Target.Advances in experimental medicine and biology · 2024Review
- A Comprehensive Analysis of the Prognostic, Immunological and Diagnostic Role of CCNF in Pan-cancer.Journal of Cancer · 2023Article
- In silico analysis of a Skp1 protein homolog from the human pathogenJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Article
- Developmental and regenerative paradigms of cilia regulated hedgehog signaling.Seminars in cell & developmental biology · 2021Review
- Folding for the Immune Synapse: CCT Chaperonin and the Cytoskeleton.Frontiers in cell and developmental biology · 2021Review
- Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplication.The Journal of cell biology · 2020Article
- The role of microRNA-148a and downstream DLGAP1 on the molecular regulation and tumor progression on human glioblastoma.Oncogene · 2019Article
- Fbxo41 Promotes Disassembly of Neuronal Primary Cilia.Scientific reports · 2019Article
- Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis.Cell cycle (Georgetown, Tex.) · 2019Article
- Caspase-mediated cleavage of the centrosomal proteins during apoptosis.Cell death & disease · 2018Article
- The Protein Neddylation Pathway in Trypanosoma brucei: FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION.The Journal of biological chemistry · 2017Article
- The benefits of local depletion: The centrosome as a scaffold for ubiquitin-proteasome-mediated degradation.Cell cycle (Georgetown, Tex.) · 2016Review
- Ubiquitin, the centrosome, and chromosome segregation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.The EMBO journal · 2015Article
- Cullin 4A and 4B ubiquitin ligases interact with γ-tubulin and induce its polyubiquitination.Molecular and cellular biochemistry · 2015Article
- Hedgehog signaling from the primary cilium to the nucleus: an emerging picture of ciliary localization, trafficking and transduction.Current opinion in genetics & development · 2013Review
- Dampened regulates the activating potency of Bicoid and the embryonic patterning outcome in Drosophila.Nature communications · 2013Article
- The centrosomal E3 ubiquitin ligase FBXO31-SCF regulates neuronal morphogenesis and migration.PloS one · 2013Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Centrosomes organize the mitotic spindle to ensure accurate segregation of the chromosomes in mitosis. The mechanism that ensures accurate duplication and separation of the centrosomes underlies the fidelity of chromosome segregation, but remains unknown. In Saccharomyces cerevisiae, entry into S phase and separation of spindle pole bodies each require CDC4 and CDC34, which encode components of an SCF (Skp1-cullin-F-box) ubiquitin ligase, but a direct (SCF) connection to the spindle pole body is unknown. Using immunofluorescence microscopy, we show that in mammalian cells the Skp1 protein and the cullin Cul1 are localized to interphase and mitotic centrosomes and to the cytoplasm and nucleus. Deconvolution and immunoelectron microscopy suggest that Skp1 forms an extended pericentriolar structure that may function to organize the centrosome. Purified centrosomes also contain Skp1, and Cul1 modified by the ubiquitin-like molecule NEDD8, suggesting a role for NEDD8 in targeting. Using an in vitro assay for centriole separation in Xenopus extracts, antibodies to Skp1 or Cul1 block separation. Proteasome inhibitors block both centriole separation in vitro and centrosome duplication in Xenopus embryos. We identify candidate centrosomal F-box proteins, suggesting that distinct SCF complexes may direct proteolysis of factors mediating multiple steps in the centrosome cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.