ArticleBritish journal of pharmacology1999
Effects of anion channel antagonists in canine colonic myocytes: comparative pharmacology of Cl-, Ca2+ and K+ currents.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- LRRC8A-containing anion channels promote glioblastoma proliferation via a WNK1/mTORC2-dependent mechanism.The Journal of physiology · 2026Article
- Mechanism of action of two potent LRRC8/VRAC channel inhibitors.Research square · 2026Article
- A SWELL time to develop the molecular pharmacology of the volume-regulated anion channel (VRAC).Channels (Austin, Tex.) · 2022Review
- Increased long QT and torsade de pointes reporting on tamoxifen compared with aromatase inhibitors.Heart (British Cardiac Society) · 2018Article
- Natural and synthetic flavonoids, novel blockers of the volume-regulated anion channels, inhibit endothelial cell proliferation.Pflugers Archiv : European journal of physiology · 2018Article
- Increase in hypotonic stress-induced endocytic activity in macrophages via ClC-3.Molecules and cells · 2014Article
- The role of K+ and Cl- channels in the regulation of retinal arteriolar tone and blood flow.Investigative ophthalmology & visual science · 2014Article
- Ano1 as a regulator of proliferation.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- The stretch-dependent potassium channel TREK-1 and its function in murine myometrium.The Journal of physiology · 2011Article
- Biophysically based modeling of the interstitial cells of cajal: current status and future perspectives.Frontiers in physiology · 2011Article
- Pathways for ATP release by bovine ciliary epithelial cells, the initial step in purinergic regulation of aqueous humor inflow.American journal of physiology. Cell physiology · 2010Article
- Article
- Ca2+-activated Cl- current in retinal arteriolar smooth muscle.Investigative ophthalmology & visual science · 2009Article
- Inhibition of a cardiac sarcoplasmic reticulum chloride channel by tamoxifen.Pflugers Archiv : European journal of physiology · 2008Article
- Differential expression of ionic conductances in interstitial cells of Cajal in the murine gastric antrum.The Journal of physiology · 2008Article
- Effects of chloride and potassium channel blockers on apoptotic cell shrinkage and apoptosis in cortical neurons.Pflugers Archiv : European journal of physiology · 2004Article
- Effects of chloride channel blockers on hypotonicity-induced contractions of the rat trachea.British journal of pharmacology · 2004Article
- Ca(2+)-activated Cl(-) current in rabbit sinoatrial node cells.The Journal of physiology · 2002Article
- ClNetherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation · 2001Review
- Effects of anti-oestrogens and beta-estradiol on calcium uptake by cardiac sarcoplasmic reticulum.British journal of pharmacology · 2001Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
1. Volume-Sensitive, Outwardly Rectifying (VSOR) Cl- currents were measured in canine colonic myocytes by whole-cell patch clamp. Decreasing extracellular osmolarity 50 milliosmoles l-1 activated current that was carried by Cl- and 5 - 7 times greater in the outward direction. 2. Niflumic acid, an inhibitor of Ca2+-activated Cl- channels, did not inhibit VSOR Cl- current. Glibenclamide, an antagonist of CFTR, and anthracene-9-carboxylate (9-AC) inhibited current less than 25% at 100 microM. 3. DIDS (4, 4-diisothiocyanato-stilbene-2,2'disulphonate) inhibited VSOR Cl- current more potently than SITS (4-acetamido-4'-isothiocyanato-stilbene-2,2'-disulphonate). IC50s were 0.84 and 226 microM, respectively. 4. VSOR Cl- current was strongly inhibited by tamoxifen ([Z]-1-[p-dimethylaminoethoxy-phenyl]-1,2-diphenyl-1-butene), an anti-oestrogen compound (IC50=0.57 microM). 5. Gd3+ antagonized VSOR Cl- current more potently than La3+. The IC50 for Gd3+ was 23 microM. In contrast, 100 microM La3+ inhibited current only 35+/-7%. 6. Antagonists of VSOR Cl- current had non-specific effects. These compounds blocked voltage-dependent K+ and Ca2+ currents in colonic myocytes. Tamoxifen (10 microM) and DIDS (10 microM) inhibited L-type Ca2+ current 87+/-7 and 31+/-5%, respectively. Additionally, in the presence of 300 nM charybdotoxin, tamoxifen (1 microM) and DIDS (10 microM) inhibited delayed rectifier K+ current 38+/-8 and 10+/-2%, respectively. 7. The pharmacology of VSOR Cl- channels overlaps with voltage-dependent cation channels. DIDS and tamoxifen inhibited VSOR Cl- equally. However, because DIDS had much less effect on L-type Ca2+ and delayed rectifier K+ channels than did tamoxifen, it might be useful in experiments to investigate the physiological and pathophysiological role of this conductance in whole tissues.
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