ArticlePlant physiology1999
Elevation of the cytosolic free [Ca2+] is indispensable for the transduction of the Nod factor signal in alfalfa.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 107 citations in OpenAlex.
- Article
- A quantitative hypermorphiceLife · 2017Article
- Nod Factor Effects on Root Hair-Specific Transcriptome of Medicago truncatula: Focus on Plasma Membrane Transport Systems and Reactive Oxygen Species Networks.Frontiers in plant science · 2016Article
- Potential regulatory phosphorylation sites in a Medicago truncatula plasma membrane proton pump implicated during early symbiotic signaling in roots.FEBS letters · 2015Article
- Calcium/Calmodulin-dependent protein kinase is negatively and positively regulated by calcium, providing a mechanism for decoding calcium responses during symbiosis signaling.The Plant cell · 2013Article
- Biochemical characteristics of the Ca2+ pumping ATPase in the peribacteroid membrane from broad bean root nodules.Protoplasma · 2013Article
- A ubiquitin ligase of symbiosis receptor kinase involved in nodule organogenesis.Plant physiology · 2012Article
- Contribution of Glomus intraradices inoculation to nutrient acquisition and mitigation of ionic imbalance in NaCl-stressed Trigonella foenum-graecum.Mycorrhiza · 2012Article
- Nuclear-localized and deregulated calcium- and calmodulin-dependent protein kinase activates rhizobial and mycorrhizal responses in Lotus japonicus.The Plant cell · 2012Article
- A novel interaction between CCaMK and a protein containing the Scythe_N ubiquitin-like domain in Lotus japonicus.Plant physiology · 2011Article
- Calcium signaling in pathogenic and beneficial plant microbe interactions: what can we learn from the interaction between Piriformospora indica and Arabidopsis thaliana.Plant signaling & behavior · 2009Review
- Lotus japonicus CASTOR and POLLUX are ion channels essential for perinuclear calcium spiking in legume root endosymbiosis.The Plant cell · 2008Article
- Fungal hypaphorine reduces growth and induces cytosolic calcium increase in root hairs of Eucalyptus globulus.Protoplasma · 2007Article
- Extracellular ATP in plants. Visualization, localization, and analysis of physiological significance in growth and signaling.Plant physiology · 2006Article
- Signals and Responses: Choreographing the Complex Interaction between Legumes and alpha- and beta-Rhizobia.Plant signaling & behavior · 2006Article
- Nod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.Plant physiology · 2005Article
- Article
- Infection and invasion of roots by symbiotic, nitrogen-fixing rhizobia during nodulation of temperate legumes.Microbiology and molecular biology reviews : MMBR · 2004Review
- A nonsymbiotic root hair tip growth phenotype in NORK-mutated legumes: implications for nodulation factor-induced signaling and formation of a multifaceted root hair pocket for bacteria.The Plant cell · 2004Article
- The distributional changes and role of microtubules in Nod factor-challenged Medicago sativa root hairs.Planta · 2003Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In root hairs of alfalfa (Medicago sativa), the requirement of Ca(2+) for Nod factor signaling has been investigated by means of ion-selective microelectrodes. Measured 50 to 100 microm behind the growing tip, 0.1 microM NodRm-IV(C16:2,S) increased the cytosolic free [Ca2+] by about 0.2 pCa, while the same concentration of chitotetraose, the nonactive glucosamine backbone, had no effect. We demonstrate that NodRm-IV(C16:2,S) still depolarized the plasma membrane at external Ca(2+) concentrations below cytosolic values if the free EGTA concentration remained low (</=0.01 mM). Externally added Sr(2+) was able to replace Ca(2+), and to some extent even enhanced the Nod-factor-induced depolarization, whereas with Mg(2+) it was decreased. This suggests that the Nod factor response is triggered by Ca(2+) from external stores. The addition of the endomembrane Ca(2+)-ATPase inhibitor 2,5-di(t-butyl)-1, 4-benzohydroquinone, which presumably mobilizes Ca(2+) from Ins(1,4, 5)P(3)-sensitive stores, mimicked the Nod factor response, i.e. increased the cytosolic free [Ca2+], triggered Cl(-)-efflux, depolarized the plasma membrane, and alkalized the root hair space. In all cases a refractory state toward Nod factor perception was produced, indicating a shortcut of Nod factor signal transduction by releasing Ca(2+) from internal stores. These latter results strongly support the idea that an elevation of cytosolic free [Ca2+] is indispensable for the transduction of the Nod factor signal, which is consistent with the role of Ca(2+) as a second messenger.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.