ArticleJournal of virology1999
Induction and maintenance of autonomous flock house virus RNA1 replication.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostability.Journal of virology · 2024Article
- Plant Expression of Trans-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Update.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Nonmonotonic Dose Dependence of Protein Expression in Cells Transfected with Self-Amplifying RNA.Journal of virology · 2022Article
- A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting inG3 (Bethesda, Md.) · 2019Article
- Article
- Nanoparticle encapsidation of Flock house virus by auto assembly of Tobacco mosaic virus coat protein.International journal of molecular sciences · 2014Article
- Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence.BMB reports · 2014Article
- Viruses and antiviral immunity in Drosophila.Developmental and comparative immunology · 2014Review
- Mitochondrion-enriched anionic phospholipids facilitate flock house virus RNA polymerase membrane association.Journal of virology · 2009Article
- The heat shock protein 70 cochaperone YDJ1 is required for efficient membrane-specific flock house virus RNA replication complex assembly and function in Saccharomyces cerevisiae.Journal of virology · 2008Article
- In vivo self-interaction of nodavirus RNA replicase protein a revealed by fluorescence resonance energy transfer.Journal of virology · 2005Article
- The cellular chaperone heat shock protein 90 facilitates Flock House virus RNA replication in Drosophila cells.Journal of virology · 2005Article
- Nodamura virus nonstructural protein B2 can enhance viral RNA accumulation in both mammalian and insect cells.Journal of virology · 2004Article
- Engineered retargeting of viral RNA replication complexes to an alternative intracellular membrane.Journal of virology · 2003Article
- Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors.Journal of virology · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing study of the kinetics of RNA1 replication in the absence of either RNA2 or capsid proteins. However, RNA1 replication ceases ca. 3 days after transfection despite the presence of replication-competent RNA. We examined this inhibition by inducing the expression of RNA1 in cells from a cDNA copy that was under the control of a hormone-regulated RNA polymerase II promoter. This system reproduced the shutoff of RNA replication when DNA-templated primary transcription was turned off. Continued primary transcription partially alleviated the shutoff and maintained the rate of RNA replication for several days at a steady-state level approximately one-third that of the peak rate. After shutoff, RNA replication could be restored by transferring the resulting intracellular RNA to fresh cells or by reinducing primary transcription, indicating that cessation of replication occurred despite the competence of both the viral RNA and the cytoplasmic environment. These data suggest that there is a mechanism by which replication is shut off at late times after transfection, which may reflect the natural endpoint of the replicative cycle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.