ArticleJournal of bacteriology1999
The Caulobacter crescentus CgtA protein displays unusual guanine nucleotide binding and exchange properties.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli.Nature communications · 2024Article
- YbiB: a novel interactor of the GTPase ObgE.Nucleic acids research · 2023Article
- The structure-function analysis of Obg-like GTPase proteins along the evolutionary tree from bacteria to humans.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- GTP Binding is Necessary for the Activation of a Toxic Mutant Isoform of the Essential GTPase ObgE.International journal of molecular sciences · 2019Article
- A Mutant Isoform of ObgE Causes Cell Death by Interfering with Cell Division.Frontiers in microbiology · 2017Article
- Article
- The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target.BMC microbiology · 2015Article
- Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes.Nucleic acids research · 2013Article
- Rbg1-Tma46 dimer structure reveals new functional domains and their role in polysome recruitment.Nucleic acids research · 2012Article
- The universally conserved prokaryotic GTPases.Microbiology and molecular biology reviews : MMBR · 2011Review
- Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis.BMC microbiology · 2011Article
- YsxC, an essential protein in Staphylococcus aureus crucial for ribosome assembly/stability.BMC microbiology · 2009Article
- AtObgC, a plant ortholog of bacterial Obg, is a chloroplast-targeting GTPase essential for early embryogenesis.Plant molecular biology · 2009Article
- The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.Molecular microbiology · 2009Article
- The growth-promoting and stress response activities of the Bacillus subtilis GTP binding protein Obg are separable by mutation.Journal of bacteriology · 2008Article
- Characterization of a novel prokaryotic GDP dissociation inhibitor domain from the G protein coupled membrane protein FeoB.Journal of molecular biology · 2008Article
- Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.Molecular and cellular biology · 2007Article
- G-protein control of the ribosome-associated stress response protein SpoT.Journal of bacteriology · 2007Article
- In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.Molecular genetics and genomics : MGG · 2007Article
- Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The Caulobacter crescentus CgtA protein is a member of the Obg-GTP1 subfamily of monomeric GTP-binding proteins. In vitro, CgtA specifically bound GTP and GDP but not GMP or ATP. CgtA bound GTP and GDP with moderate affinity at 30 degrees C and displayed equilibrium binding constants of 1.2 and 0.5 microM, respectively, in the presence of Mg(2+). In the absence of Mg(2+), the affinity of CgtA for GTP and GDP was reduced 59- and 6-fold, respectively. N-Methyl-3'-O-anthranoyl (mant)-guanine nucleotide analogs were used to quantify GDP and GTP exchange. Spontaneous dissociation of both GDP and GTP in the presence of 5 to 12 mM Mg(2+) was extremely rapid (k(d) = 1.4 and 1.5 s(-1), respectively), 10(3)- to 10(5)-fold faster than that of the well-characterized eukaryotic Ras-like GTP-binding proteins. The dissociation rate constant of GDP increased sevenfold in the absence of Mg(2+). Finally, there was a low inherent GTPase activity with a single-turnover rate constant of 5.0 x 10(-4) s(-1) corresponding to a half-life of hydrolysis of 23 min. These data clearly demonstrate that the guanine nucleotide binding and exchange properties of CgtA are different from those of the well-characterized Ras-like GTP-binding proteins. Furthermore, these data are consistent with a model whereby the nucleotide occupancy of CgtA is controlled by the intracellular levels of guanine nucleotides.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.