ArticleJournal of bacteriology1999
Bacterial conjugation protein MobA mediates integration of complex DNA structures into plant cells.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- A Novel ARTP-derived Bacillus megaterium Mutant with Enhanced Salt Tolerance and Plant Growth Promotion in Saline-alkali Soil.Current microbiology · 2026Article
- Oxytetracycline and Streptomycin Resistance Genes inFrontiers in microbiology · 2022Article
- Successful Transfer of a Model T-DNA Plasmid toFrontiers in microbiology · 2018Article
- Nuclear targeting of a bacterial integrase that mediates site-specific recombination between bacterial and human target sequences.Applied and environmental microbiology · 2011Article
- Transposition-based plant transformation.Plant physiology · 2007Article
- Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.Biochimica et biophysica acta · 2004Review
- Agrobacterium tumefaciens-mediated transformation of plants by the pTF-FC2 plasmid is efficient and strictly dependent on the MobA protein.Plant molecular biology · 2004Article
- Mobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1.Journal of bacteriology · 2001Article
- Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens transfers T-DNA to plant cells, where it integrates into the genome, a property that is ensured by bacterial proteins VirD2 and VirE2. Under natural conditions, the protein MobA mobilizes its encoding plasmid, RSF1010, between different bacteria. A detailed analysis of MobA-mediated DNA mobilization by Agrobacterium to plants was performed. We compared the ability of MobA to transfer DNA and integrate it into the plant genome to that of pilot protein VirD2. MobA was found to be about 100-fold less efficient than VirD2 in conducting the DNA from the pTi plasmid to the plant cell nucleus. However, interestingly, DNAs transferred by the two proteins were integrated into the plant cell genome with similar efficiencies. In contrast, most of the integrated DNA copies transferred from a MobA-containing strain were truncated at the 5' end. Isolation and analysis of the most conserved 5' ends revealed patterns which resulted from the illegitimate integration of one transferred DNA within another. These complex integration patterns indicate a specific deficiency in MobA. The data conform to a model according to which efficiency of T-DNA integration is determined by plant enzymes and integrity is determined by bacterial proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.