ArticleJournal of bacteriology1999
Involvement of the cis/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-T1E.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 183 citations in OpenAlex.
- SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.Engineering in life sciences · 2025Article
- Unraveling the genomic diversity of the Pseudomonas putida group: exploring taxonomy, core pangenome, and antibiotic resistance mechanisms.FEMS microbiology reviews · 2024Review
- Employing Metadynamics to Predict the Membrane Partitioning of Carboxy-2The journal of physical chemistry. B · 2024Article
- Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas.Microbial cell factories · 2024Article
- Insights into the susceptibility of Pseudomonas putida to industrially relevant aromatic hydrocarbons that it can synthesize from sugars.Microbial cell factories · 2023Article
- Providing octane degradation capability to Pseudomonas putida KT2440 through the horizontal acquisition of oct genes located on an integrative and conjugative element.Environmental microbiology reports · 2022Article
- Effect of Culture Conditions on Fatty Acid Profiles of Bacteria and Lipopolysaccharides of the GenusMolecules (Basel, Switzerland) · 2022Article
- Adaptation of Pseudomonas helmanticensis to fat hydrolysates and SDS: fatty acid response and aggregate formation.Journal of microbiology (Seoul, Korea) · 2021Article
- Characterizing Escherichia coli's transcriptional response to different styrene exposure modes reveals novel toxicity and tolerance insights.Journal of industrial microbiology & biotechnology · 2021Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- A novel synthesis of trans-unsaturated fatty acids by the Gram-positive commensal bacterium Enterococcus faecalis FA2-2.Chemistry and physics of lipids · 2019Article
- Transcriptional regulation of fatty acid cis-trans isomerization in the solvent-tolerant soil bacterium, Pseudomonas putida F1.Environmental microbiology · 2019Article
- The Various Roles of Fatty Acids.Molecules (Basel, Switzerland) · 2018Review
- Membrane engineering via trans-unsaturated fatty acids production improves succinic acid production in Mannheimia succiniciproducens.Journal of industrial microbiology & biotechnology · 2018Article
- Immediate response mechanisms of Gram-negative solvent-tolerant bacteria to cope with environmental stress: cis-trans isomerization of unsaturated fatty acids and outer membrane vesicle secretion.Applied microbiology and biotechnology · 2018Review
- A Broad Spectrum Racemase inFrontiers in microbiology · 2018Article
- Engineering membrane and cell-wall programs for tolerance to toxic chemicals: Beyond solo genes.Current opinion in microbiology · 2016Review
- Making variability less variable: matching expression system and host for oxygenase-based biotransformations.Journal of industrial microbiology & biotechnology · 2015Article
- Response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane.International journal of microbiology · 2014Review
- Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome.Microbial biotechnology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholipids, mainly cis-oleic acid (C(16:1,9)) and cis-vaccenic acid (C(18:1,11)), in response to solvents. To determine the importance of this cis/trans isomerase for solvent resistance a Cti-null mutant was generated and characterized. This mutant showed a longer lag phase when grown with toluene in the vapor phase; however, after the lag phase the growth rate of the mutant strain was similar to that of the wild type. The mutant also showed a significantly lower survival rate when shocked with 0.08% (vol/vol) toluene. In contrast to the wild-type strain, which grew in liquid culture medium at temperatures up to 38.5 degrees C, the Cti-null mutant strain grew significantly slower at temperatures above 37 degrees C. An in-frame fusion of the Cti protein with the periplasmic alkaline phosphatase suggests that this constitutively expressed enzyme is located in the periplasm. Primer extension studies confirmed the constitutive expression of Cti. Southern blot analysis of total DNA from various pseudomonads showed that the cti gene is present in all the tested P. putida strains, including non-solvent-resistant ones, and in some other Pseudomonas species.
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