ArticleTransgenic research1999
Type II fish antifreeze protein accumulation in transgenic tobacco does not confer frost resistance.
Article in Transgenic research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 51 citations in OpenAlex.
- Cold-inducible promoter-driven knockdown ofPlant direct · 2022Article
- Cold adaptation strategies in plants-An emerging role of epigenetics and antifreeze proteins to engineer cold resilient plants.Frontiers in genetics · 2022Review
- A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering.3 Biotech · 2019Review
- Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana.Plant biotechnology journal · 2017Article
- Ice-Binding Proteins in Plants.Frontiers in plant science · 2017Review
- Antifreeze proteins enable plants to survive in freezing conditions.Journal of biosciences · 2014Review
- Antifreeze peptides and glycopeptides, and their derivatives: potential uses in biotechnology.Marine drugs · 2013Review
- Ice recrystallization inhibition proteins of perennial ryegrass enhance freezing tolerance.Planta · 2010Article
- Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco.Plant cell reports · 2008Article
- Plants in a cold climate.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2002Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type II fish antifreeze protein (AFP) is active in both freezing point depression and the inhibition of ice recrystallization. This extensively disulfide-bonded 14 kDa protein was targeted for accumulation in its pro- and mature forms in the cytosol and apoplast of transgenic tobacco plants. Type II AFP gene constructs under control of a duplicate cauliflower mosaic virus 35S promoter, both with and without a native plant transit peptide sequence, were introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. AFP did not accumulate in the cytosol of transgenic plants, but active AFP was present as 2% the total protein present in the apoplast. Plant-produced AFP was the same size as mature Type II AFP isolated from fish, and was comparable to wild-type AFP in thermal hysteresis activity and its effect on ice crystal morphology. Field trials conducted in late summer on R1 generation transgenic plants showed similar AFP accumulation in plants under field conditions at levels suitable for large-scale production: but no difference in frost resistance was observed between transgenic and wild-type plants during the onset of early fall frosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.