ArticlePlant molecular biology1999
Transcript accumulation and utilization of alternate and non-consensus splice sites in rice granule-bound starch synthase are temperature-sensitive and controlled by a single-nucleotide polymorphism.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 160 citations in OpenAlex.
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- Effect of Heading Date on the Starch Structure and Grain Yield of Rice Lines with Low Gelatinization Temperature.International journal of molecular sciences · 2022Article
- Improving Agricultural Traits While Maintaining High Resistant Starch Content in Rice.Rice (New York, N.Y.) · 2022Article
- Targeted Deletion of the First Intron of the WxRice (New York, N.Y.) · 2022Review
- Non-destructive monitoring of amylose content in rice by UAV-based hyperspectral images.Frontiers in plant science · 2022Article
- Comparative Phosphoproteomic Analysis Reveals the Response of Starch Metabolism to High-Temperature Stress in Rice Endosperm.International journal of molecular sciences · 2021Article
- Global Survey of Alternative Splicing in Rice by Direct RNA Sequencing During Reproductive Development: Landscape and Genetic Regulation.Rice (New York, N.Y.) · 2021Article
- Reading between the Lines: RNA-seq Data Mining Reveals the Alternative Message of the Rice Leaf Transcriptome in Response to Heat Stress.Plants (Basel, Switzerland) · 2021Article
- Review
- Genetic Control and High Temperature Effects on Starch Biosynthesis and Grain Quality in Rice.Frontiers in plant science · 2021Review
- Article
- Haplotype Variations and Evolutionary Analysis of the Granule-Bound Starch Synthase I Gene in the Korean World Rice Collection.Frontiers in plant science · 2021Article
- Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China.PloS one · 2020Article
- Integrating a genome-wide association study with a large-scale transcriptome analysis to predict genetic regions influencing the glycaemic index and texture in rice.Plant biotechnology journal · 2019Article
- The qSAC3 locus from indica rice effectively increases amylose content under a variety of conditions.BMC plant biology · 2019Article
- Association Analysis of Three Diverse Rice (The plant genome · 2019Article
- Variation in composition, protein and pasting characteristics of different pigmented and non pigmented rice (Journal of food science and technology · 2018Article
- Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities.BMC plant biology · 2018Article
- Suppression of OsMADS7 in rice endosperm stabilizes amylose content under high temperature stress.Plant biotechnology journal · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Granule-bound starch synthase (GBSS), a product of the waxy gene in rice (Oryza sativa L.), is necessary for the synthesis of amylose in the endosperm. In an extended pedigree of 89 rice cultivars, we have previously shown that all cultivars with more than 18% amylose had the sequence AGGTATA at the leader intron 5' splice site, while all cultivars with a lower proportion of amylose had the sequence AGTTATA. This single-nucleotide polymorphism reduces the efficiency of GBSS pre-mRNA processing. It also results in alternate splicing at multiple sites, some of which have non-consensus sequences. Here we demonstrate that this same G-to-T polymorphism is also associated with differential sensitivity to temperature during the period of grain development. Cultivars with the sequence AGTTATA have a substantial increase in accumulation of mature GBSS transcripts at 18 degrees C compared to 25 or 32 degrees C. The selection of leader intron 5' splice sites is also affected by temperature in these cultivars. A 5' splice site -93 upstream from that used in high-amylose varieties predominates at 18 degrees C. At higher temperatures there is increased utilization of a 5' splice site at -I and a non-consensus site at +1. Potential implications of differential 5' splice site selection and associated differences in 3' splice site selection on transcript stability and translational efficiency are discussed.
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