ArticlePlant molecular biology1999
Interaction between composite elements in the napA promoter: both the B-box ABA-responsive complex and the RY/G complex are necessary for seed-specific expression.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 161 citations in OpenAlex.
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- Functional characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean.Genes · 2023Article
- Meta-QTL and haplo-pheno analysis reveal superior haplotype combinations associated with low grain chalkiness under high temperature in rice.Frontiers in plant science · 2023Article
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- The chloroplast redox-responsive transcriptome of solanaceous plants reveals significant nuclear gene regulatory motifs associated to stress acclimation.Plant molecular biology · 2022Article
- Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.PloS one · 2022Article
- Genome-wide prediction of cauliflower miRNAs and lncRNAs and their roles in post-transcriptional gene regulation.Planta · 2021Article
- Genome-wide identification and expression profiles of ERF subfamily transcription factors inPeerJ · 2020Article
- Characteristics and Expression Pattern ofPlants (Basel, Switzerland) · 2019Article
- Isolation and characterization of a novel seed-specific promoter from peanut (Arachis hypogaea L.).Molecular biology reports · 2019Article
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- Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.Plant cell reports · 2018Article
- Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.Plant biotechnology journal · 2018Article
- The linin promoter is highly effective in enhancing punicic acid production in Arabidopsis.Plant cell reports · 2017Article
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- Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat.Frontiers in plant science · 2017Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During seed maturation, the transcriptional activity of napin genes is regulated by developmental signals involving the transcriptional activator ABI3 and abscisic acid (ABA). To localize cis elements involved in the seed-specific activity of the napin napA promoter, a systematic analysis was performed focusing on two major element complexes, the B-box and RY/G. Substitution mutation analysis using promoter-reporter gene fusions in stable transgenic tobacco showed synergistic interactions between elements within these complexes. The distal part of the B-box shows similarities to abscisic acid response elements and the proximal portion contains a CA-rich element. In vitro studies involving Exonuclease III protection and electrophoretic mobility shift assays revealed binding by nuclear proteins to elements within the B-box. The distal and proximal parts of the B-box were found to bind distinct nuclear protein complexes. By gain-of-function analysis with a tetramer of the B-box fused to a truncated (-46) cauliflower mosaic virus (CaMV) 35S minimal promoter, it was demonstrated that the B-box mediates strong activity in seeds. Further, it was shown that the elements in the B-box constitute an ABA-responsive complex, since the B-box tetramer mediates ABA-responsiveness in vegetative tissues to a construct containing the CaMV virus 35S enhancer (-343 to -90). Thus, the seed-specific activity of the napA promoter relies on the combinatorial interaction between the RY/G complex and the B-box ABA-responsive complex during the ABA response in seed development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.