ArticlePlant molecular biology1999
Antisense suppression of tomato endo-1,4-beta-glucanase Cel2 mRNA accumulation increases the force required to break fruit abscission zones but does not affect fruit softening.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 166 citations in OpenAlex.
- The novel function of the bZIP transcription factor VSF-1 in mediating fruit firmness in tomato.Horticulture research · 2026Article
- Expression patterns of Arabidopsis endo-β-1,4-glucanases and their putative roles in sexual reproduction.BMC plant biology · 2026Article
- Proteome and Phosphoproteome of Tomato Fruit Identify REDUCED CHLOROPLAST COVERAGE 1a as A Ripening Regulator.Genomics, proteomics & bioinformatics · 2025Article
- Abscission zones: cellular interfaces for the programmed separation of organs.Annals of botany · 2025Review
- ENDOGLUCANASE SlCEL2 and EXPANSIN SlEXP1 synergistically affect cellulose degrading and tomato fruit softening.BMC plant biology · 2025Article
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato.The Plant cell · 2024Article
- Genome-wide analysis of the mulberry (Frontiers in plant science · 2024Article
- Genome-wide identification of GH9 gene family and the assessment of its role during fruit abscission zone formation in Vaccinium ashei.Plant cell reports · 2023Article
- Insights into cell wall changes during fruit softening from transgenic and naturally occurring mutants.Plant physiology · 2023Review
- Genome-Wide Characteristics of GH9B Family Members in Melon and Their Expression Profiles under Exogenous Hormone and Far-Red Light Treatment during the Grafting Healing Process.International journal of molecular sciences · 2023Article
- Grain shattering by cell death and fracture in Eragrostis tef.Plant physiology · 2023Article
- Low Temperature Inhibits the Defoliation Efficiency of Thidiazuron in Cotton by Regulating Plant Hormone Synthesis and the Signaling Pathway.International journal of molecular sciences · 2022Article
- Article
- Genome Wide Identification and Characterization of WheatInternational journal of molecular sciences · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall ofMetabolites · 2021Article
- A tomato LATERAL ORGAN BOUNDARIES transcription factor,Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Tomato SlBL4 plays an important role in fruit pedicel organogenesis and abscission.Horticulture research · 2021Article
- Transcriptome Responses of Ripe Cherry Tomato Fruit Exposed to Chilling and Rewarming Identify Reversible and Irreversible Gene Expression Changes.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants of tomato (Lycopersicon esculentum Mill. cv. T5) were transformed with an antisense endo-1,4-beta-glucanase (cellulase, EC 3.2.1.4) Cel2 transgene under the control of the constitutive cauliflower mosaic virus 35S promoter in order to suppress mRNA accumulation of Cel2. In two independent transgenic lines, Cel2 mRNA abundance was reduced by >95% in ripe fruit pericarp and ca. 80% in fruit abscission zones relative to non-transgenic controls. In both transgenic lines the softening of antisense Cel2 fruit pericarp measured using stress-relaxation analysis was indistinguishable from control fruit. No differences in ethylene evolution were observed between fruit of control and antisense Cel2 genotypes. However, in fruit abscission zones the suppression of Cel2 mRNA accumulation caused a significant (P<0.001) increase in the force required to cause breakage of the abscission zone at 4 days post breaker, an increase of 27% in one transgenic line and of 46% in the other transgenic line. Thus the Cel2 gene product contributes to cell wall disassembly occurring in cell separation during fruit abscission, but its role, if any, in softening or textural changes occurring in fruit pericarp during ripening was not revealed by suppression of Cel2 gene expression.
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