ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Measurement of action potential-induced presynaptic calcium domains at a cultured neuromuscular junction.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 89 citations in OpenAlex.
- Homeostatic preservation of action potential waveform by Notch and Nerfin-1.Current biology : CB · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.Research square · 2025Article
- Spatial and temporal aspects of neuronal calcium and sodium signals measured with low-affinity fluorescent indicators.Pflugers Archiv : European journal of physiology · 2024Review
- Lytic granule exocytosis at immune synapses: lessons from neuronal synapses.Frontiers in immunology · 2023Review
- Roles and Sources of Calcium in Synaptic Exocytosis.Advances in neurobiology · 2023Article
- Functional Microstructure of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Functional Specialization of Interneuron Dendrites: Identification of Action Potential Initiation Zone in Axonless Olfactory Bulb Granule Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- EGTA Can Inhibit Vesicular Release in the Nanodomain of Single CaFrontiers in synaptic neuroscience · 2019Article
- Quantitative optical nanophysiology of CaNature communications · 2018Article
- Mechanisms of Supralinear Calcium Integration in Dendrites of Hippocampal CA1 Fast-Spiking Cells.Frontiers in synaptic neuroscience · 2018Article
- Location matters: somatic and dendritic SK channels answer to distinct calcium signals.Journal of neurophysiology · 2015Article
- Reduced endogenous Ca2+ buffering speeds active zone Ca2+ signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Synaptic activity regulates the abundance and binding of complexin.Biophysical journal · 2015Article
- Ca2+ channel to synaptic vesicle distance accounts for the readily releasable pool kinetics at a functionally mature auditory synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Nanoscale distribution of presynaptic Ca(2+) channels and its impact on vesicular release during development.Neuron · 2015Article
- Advances in imaging ultrastructure yield new insights into presynaptic biology.Frontiers in cellular neuroscience · 2015Review
- Article
- Transients in global Ca2+ concentration induced by electrical activity in a giant nerve terminal.The Journal of physiology · 2013Review
- Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.Journal of visualized experiments : JoVE · 2013Article
- Sustaining rapid vesicular release at active zones: potential roles for vesicle tethering.Trends in neurosciences · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spatially localized Ca(2+) domains are thought to play a key role in action potential (AP)-evoked neurotransmitter release at fast synapses. We used a stage-scan confocal spot-detection method and the low-affinity Ca(2+) indicator Oregon Green 488 BAPTA-5N to study the spatiotemporal profile of presynaptic AP-induced Ca(2+) domains. Families of scanned AP-induced fluorescence transients were detected from spot locations separated by 200-300 nm, within the vicinity of Ca(2+) entry sites. Typically, the largest transient in a particular scan peaked within approximately 1 msec and decayed with rapid (tau(1) of 1.7 msec) and slow components (tau(2) of 16 msec, tau(3) of 78 msec). As the spot was incrementally displaced, transients progressively exhibited a slowing in their time-to-peak and a loss of the fast decay component. Three-dimensional graphs of fluorescence versus time and spot displacement revealed the presence of AP-induced fluorescence domains that dissipated within approximately 7 msec. The size of fluorescence domains were estimated from the full-width at half-maximum of gaussian fits to isochronal DeltaF/F plots and ranged from 0.6 to 3.0 micrometer, with a mean +/- SD of 1.6 +/- 0.6 micrometer. Model simulations of a localized Ca(2+) entry site predicted the major features of the fluorescence transients and suggested that, within approximately 1 msec of the initiation of the Ca(2+) current, both the fluorescence domain and the underlying Ca(2+) domain do not extend significantly beyond the site of entry. Consistent with this prediction, the intracellular addition of EGTA (up to 2 mM) accelerated the decay of the measured transients but did not affect the domain size.
Indexed as
Identifiers
10479687PMC6782444W2108204473What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.