ArticleIn vitro cellular & developmental biology. Animal1999
Differentiation markers of mouse C2C12 and rat L6 myogenic cell lines and the effect of the differentiation medium.
Article in In vitro cellular & developmental biology. Animal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
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- Variability in Cardiac miRNA-122 Level Determines Therapeutic Potential of miRNA-Regulated AAV Vectors.Molecular therapy. Methods & clinical development · 2020Article
- Bringing cultured meat to market: Technical, socio-political, and regulatory challenges in cellular agriculture.Trends in food science & technology · 2018Review
- Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine.Data in brief · 2016Article
- Lipoic Acid Exerts Antioxidant and Anti-inflammatory Effects in Response to Heat Shock in C2C12 Myotubes.Inflammation · 2016Article
- Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease.Skeletal muscle · 2016Article
- Combining docking site and phosphosite predictions to find new substrates: identification of smoothelin-like-2 (SMTNL2) as a c-Jun N-terminal kinase (JNK) substrate.Cellular signalling · 2013Article
- Identification of retinoic acid in a high content screen for agents that overcome the anti-myogenic effect of TGF-beta-1.PloS one · 2010Article
- Mechanism and regulation of acetylated histone binding by the tandem PHD finger of DPF3b.Nature · 2010Article
- Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells.Differentiation; research in biological diversity · 2009Article
- Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments.The Journal of cell biology · 2004Article
- Heparan sulfate heterogeneity in skeletal muscle basal lamina: demonstration by phage display-derived antibodies.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentiation grade of cells in culture is dependent on the composition of the culture medium. Two commonly used myogenic cell lines, mouse C2C12 and rat L6, usually differentiate at a low concentration of horse serum. In this study we compared the effect of horse serum with a medium containing a low percentage of Ultroser G and rat brain extract. The maturation grade was evaluated on the basis of various biochemical, (immuno)histochemical and cell-physiological parameters. Substitution of horse serum by Ultroser G and rat brain extract during the differentiation phase resulted in a higher maturation grade of the myotubes of both cell lines, on the basis of creatine kinase activity and the diameter of the myotubes. In addition, the C2C12 myotubes display cross-striation, contain a higher percentage of creatine kinase muscle-specific isoenzyme MM, show a ninefold increase in acetylcholine receptor (AChR) clusters, form a continuous basement membrane, and have a lower resting cytosolic Ca2+ concentration. L6 myotubes show a fivefold increase in AChR clusters and a twofold increase in the expression of the mRNA of the epsilon-subunit of AChR.C2C12 cells show spontaneous contraction and response of cytosolic Ca2+ to various stimulants in contrast to L6 cells which do not. These studies established that the Ultroser G/brain extract medium leads to a higher differentiation grade of both cell lines, but parameters appropriate for use as differentiation markers appear to differ between both cell lines.
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