ArticleThe Journal of cell biology1999
Localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- The intrinsically disordered protein SPE-18 promotes localized assembly of MSP inDevelopment (Cambridge, England) · 2021Article
- MFP1/MSD-1 and MFP2/NSPH-2 co-localize with MSP duringmicroPublication biology · 2021Article
- Concise Language Promotes Clear Thinking about Cell Shape and Locomotion.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Article
- Article
- Megakaryopoiesis and platelet production: insight into hematopoietic stem cell proliferation and differentiation.Stem cell investigation · 2015Review
- Extending the molecular clutch beyond actin-based cell motility.New journal of physics · 2014Article
- SLC6 family transporter SNF-10 is required for protease-mediated activation of sperm motility in C. elegans.Developmental biology · 2014Article
- Mathematical modeling of eukaryotic cell migration: insights beyond experiments.Annual review of cell and developmental biology · 2013Review
- Pluripotent stem cells reveal the developmental biology of human megakaryocytes and provide a source of platelets for clinical application.Cellular and molecular life sciences : CMLS · 2012Review
- Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction.Current biology : CB · 2011Article
- Protons make tumor cells move like clockwork.Pflugers Archiv : European journal of physiology · 2009Review
- Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm.Molecular biology of the cell · 2009Article
- Cofilin is a pH sensor for actin free barbed end formation: role of phosphoinositide binding.The Journal of cell biology · 2008Article
- Depolymerization-driven flow in nematode spermatozoa relates crawling speed to size and shape.Biophysical journal · 2008Article
- Exclusion of a proton ATPase from the apical membrane is associated with cell polarity and tip growth in Nicotiana tabacum pollen tubes.The Plant cell · 2008Article
- Nematode sperm motility: nonpolar filament polymerization mediated by end-tracking motors.Biophysical journal · 2007Article
- A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis.The Journal of cell biology · 2005Article
- A Simple 1-D Physical Model for the Crawling Nematode Sperm Cell.Journal of statistical physics · 2003Article
- Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene.Genetics · 2002Article
- Acting like actin. The dynamics of the nematode major sperm protein (msp) cytoskeleton indicate a push-pull mechanism for amoeboid cell motility.The Journal of cell biology · 2000Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The major sperm protein (MSP)-based amoeboid motility of Ascaris suum sperm requires coordinated lamellipodial protrusion and cell body retraction. In these cells, protrusion and retraction are tightly coupled to the assembly and disassembly of the cytoskeleton at opposite ends of the lamellipodium. Although polymerization along the leading edge appears to drive protrusion, the behavior of sperm tethered to the substrate showed that an additional force is required to pull the cell body forward. To examine the mechanism of cell body movement, we used pH to uncouple cytoskeletal polymerization and depolymerization. In sperm treated with pH 6.75 buffer, protrusion of the leading edge slowed dramatically while both cytoskeletal disassembly at the base of the lamellipodium and cell body retraction continued. At pH 6.35, the cytoskeleton pulled away from the leading edge and receded through the lamellipodium as its disassembly at the cell body continued. The cytoskeleton disassembled rapidly and completely in cells treated at pH 5.5, but reformed when the cells were washed with physiological buffer. Cytoskeletal reassembly occurred at the lamellipodial margin and caused membrane protrusion, but the cell body did not move until the cytoskeleton was rebuilt and depolymerization resumed. These results indicate that cell body retraction is mediated by tension in the cytoskeleton, correlated with MSP depolymerization at the base of the lamellipodium.
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Registered trials
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