ArticleThe Biochemical journal1999
Iron-dependent regulation of transferrin receptor expression in Trypanosoma brucei.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Genetic resistance to vector-borne hemoprotozoa in livestock: molecular markers, host-parasite interactions, and implications for breeding and control.Journal of veterinary science · 2025Review
- Increased Trypanocidal Activity of the Salinomycin Derivative Ironomycin Is Due to ROS Production and Iron Uptake Impairment.Molecules (Basel, Switzerland) · 2024Article
- Multiparameter ranking of carbazoles for anti-trypanosome lead discovery.Frontiers in drug discovery · 2024Article
- A transferrin receptor's guide to African trypanosomes.Cell surface (Amsterdam, Netherlands) · 2023Review
- Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosoma brucei.PLoS pathogens · 2021Article
- Article
- Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei.PLoS pathogens · 2018Article
- Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3'UTR.PloS one · 2018Article
- Strategies of Intracellular Pathogens for Obtaining Iron from the Environment.BioMed research international · 2015Review
- Iron Homeostasis and Trypanosoma brucei Associated Immunopathogenicity Development: A Battle/Quest for Iron.BioMed research international · 2015Review
- The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes.Eukaryotic cell · 2014Article
- Pathways of iron acquisition and utilization in Leishmania.Current opinion in microbiology · 2013Review
- Evidence that transport of iron from the lysosome to the cytosol in African trypanosomes is mediated by a mucolipin orthologue.Molecular microbiology · 2013Article
- The trypanocidal effect of NO-releasing agents is not due to inhibition of the major cysteine proteinase in Trypanosoma brucei.Parasitology research · 2009Article
- Iron trafficking as an antimicrobial target.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Review
- Proteomic and network analysis characterize stage-specific metabolism in Trypanosoma cruzi.BMC systems biology · 2009Article
- The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages.BMC genomics · 2008Article
- Two isoforms of a divalent metal transporter (DMT1) in Schistosoma mansoni suggest a surface-associated pathway for iron absorption in schistosomes.The Journal of biological chemistry · 2006Article
- Developmental variation in Rab11-dependent trafficking in Trypanosoma brucei.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transferrin is an essential growth factor for African trypanosomes. Here we show that expression of the trypanosomal transferrin receptor, which bears no structural similarity with mammalian transferrin receptors, is regulated by iron availability. Iron depletion of bloodstream forms of Trypanosoma brucei with the iron chelator deferoxamine resulted in a 3-fold up-regulation of the transferrin receptor and a 3-fold increase of the transferrin uptake rate. The abundance of expression site associated gene product 6 (ESAG6) mRNA, which encodes one of the two subunits of the trypanosome transferrin receptor, is regulated 5-fold by a post-transcriptional mechanism. In mammalian cells the stability of transferrin receptor mRNA is controlled by iron regulatory proteins (IRPs) binding to iron-responsive elements (IREs) in the 3'-untranslated region (UTR). Therefore, the role of a T. brucei cytoplasmic aconitase (TbACO) that is highly related to mammalian IRP-1 was investigated. Iron regulation of the transferrin receptor was found to be unaffected in Deltaaco::NEO/Deltaaco::HYG null mutants generated by targeted disruption of the TbACO gene. Thus, the mechanism of post-transcriptional transferrin receptor regulation in trypanosomes appears to be distinct from the IRE/IRP paradigm. The transferrin uptake rate was also increased when trypanosomes were transferred from medium supplemented with foetal bovine serum to medium supplemented with sera from other vertebrates. Due to varying binding affinities of the trypanosomal transferrin receptor for transferrins of different species, serum change can result in iron starvation. Thus, regulation of transferrin receptor expression may be a fast compensatory mechanism upon transmission of the parasite to a new host species.
Indexed as
Identifiers
10477281PMC1220511W2058145203What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.