ArticleThe Biochemical journal1999
Characterization and polyanion-binding properties of purified recombinant prion protein.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- A tetracationic porphyrin with dual anti-prion activity.iScience · 2023Article
- Unfolded and intermediate states of PrP play a key role in the mechanism of action of an antiprion chaperone.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Antisense oligonucleotides extend survival of prion-infected mice.JCI insight · 2019Article
- A nine amino acid domain is essential for mutant prion protein toxicity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Na+/K+-ATPase is present in scrapie-associated fibrils, modulates PrP misfolding in vitro and links PrP function and dysfunction.PloS one · 2011Article
- Green fluorescent protein as a reporter of prion protein folding.Virology journal · 2006Article
- Inhibitors of the mitogen-activated protein kinase kinase 1/2 signaling pathway clear prion-infected cells from PrPSc.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Distance of sequons to the C-terminus influences the cellular N-glycosylation of the prion protein.The Biochemical journal · 2003Article
- Cell-surface prion protein interacts with glycosaminoglycans.The Biochemical journal · 2002Article
- Identification of interaction domains of the prion protein with its 37-kDa/67-kDa laminin receptor.The EMBO journal · 2001Article
- Scrapie strains maintain biological phenotypes on propagation in a cell line in culture.The EMBO journal · 2001Article
- Membrane topology influences N-glycosylation of the prion protein.The EMBO journal · 2001Article
- Sulfated glycans and elevated temperature stimulate PrP(Sc)-dependent cell-free formation of protease-resistant prion protein.The EMBO journal · 2001Article
- Pentosan polysulfate regulates scavenger receptor-mediated, but not fluid-phase, endocytosis in immortalized cerebral endothelial cells.Cellular and molecular neurobiology · 2000Article
- Post-translational hydroxylation at the N-terminus of the prion protein reveals presence of PPII structure in vivo.The EMBO journal · 2000Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Certain polysulphated polyanions have been shown to have prophylactic effects on the progression of transmissible spongiform encephalopathy disease, presumably because they bind to prion protein (PrP). Until now, the difficulty of obtaining large quantities of native PrP has precluded detailed studies of these interactions. We have over-expressed murine recombinant PrP (recPrP), lacking its glycophosphoinositol membrane anchor, in modified mammalian cells. Milligram quantities of secreted, soluble and partially glycosylated protein were purified under non-denaturing conditions and the identities of mature-length aglycosyl recPrP and two cleavage fragments were determined by electrospray MS. Binding was assessed by surface plasmon resonance techniques using both direct and competitive ligand-binding approaches. recPrP binding to immobilized polyanions was enhanced by divalent metal ions. Polyanion binding was strong and showed complex association and dissociation kinetics that were consistent with ligand-directed recPrP aggregation. The differences in the binding strengths of recPrP to pentosan polysulphate and to other sulphated polyanions were found to parallel their in vivo anti-scrapie and in vitro anti-scrapie-specific PrP formation potencies. When recPrP was immobilized by capture on metal-ion chelates it was found, contrary to expectation, that the addition of polyanions promoted the dissociation of the protein.
Indexed as
Identifiers
10477271PMC1220501W1988163014What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.