ArticleIn vitro cellular & developmental biology. Animal1999
A stromal cell line from rainbow trout spleen, RTS34ST, that supports the growth of rainbow trout macrophages and produces conditioned medium with mitogenic effects on leukocytes.
Article in In vitro cellular & developmental biology. Animal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.In vitro cellular & developmental biology. Animal · 2017Review
- Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus.F1000Research · 2017Article
- B Cell-Activating Factor Regulates Different Aspects of B Cell Functionality and Is Produced by a Subset of Splenic B Cells in Teleost Fish.Frontiers in immunology · 2017Article
- Senescence-associated β-galactosidase staining in fish cell lines and primary cultures from several tissues and species, including rainbow trout coelomic fluid and milt.In vitro cellular & developmental biology. Animal · 2015Article
- Characterization of novel precursor miRNAs using next generation sequencing and prediction of miRNA targets in Atlantic halibut.PloS one · 2013Article
- Electric pulses to prepare feeder cells for sustaining and culturing of undifferentiated embryonic stem cells.Biotechnology journal · 2010Article
- Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos.Stem cells and development · 2008Article
- Homologous recombination in zebrafish ES cells.Transgenic research · 2006Article
- Characterization of rainbow trout cell lines using microsatellite DNA profiling.Cytotechnology · 2001Article
- Production of zebrafish germ-line chimeras from embryo cell cultures.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rainbow trout spleen cell line, RTS34, was developed from a long-term hemopoietic culture. This cell line consisted of a mixed stromal cell layer with an associated cell population of macrophage-like cells that formed proliferative foci and released nonadherent progeny cells into the culture medium. A stromal cell line, RTS34st, was isolated from the RTS34 cell line. RTS34st cultures contained cells with fibroblast-like and epithelial-like morphologies and showed enhanced [3H]thymidine incorporation in response to either FBS or rainbow trout serum. The combination of FBS and trout serum was synergistic. Conditioned medium from RTS34st stimulated thymidine incorporation by peripheral blood and head kidney leukocytes, but not by leukocytes from the spleen. In addition, RTS34st provided a hemopoietic inductive microenvironment for immature precursor cells, selectively supporting the growth of macrophage-like cells. Therefore, RTS34st appears useful for studying the different roles of the stroma in regulating hemopoiesis in fish.
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