ArticleMolecular pathology : MP1999
Detection of clonal B cells in microdissected reactive lymphoproliferations: possible diagnostic pitfalls in PCR analysis of immunoglobulin heavy chain gene rearrangement.
Article in Molecular pathology : MP, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Highly atypical lymphoid proliferation after COVID-19 vaccine, with spontaneous regression.Journal of hematopathology · 2024Article
- Cytologic and Molecular Diagnostics for Vitreoretinal Lymphoma: Current Approaches and Emerging Single-Cell Analyses.Frontiers in molecular biosciences · 2020Review
- The frequency of immunoglobulin heavy chain gene and T-cell receptor gamma-chain gene rearrangements and Epstein-Barr virus in ALK+ and ALK- anaplastic large cell lymphoma and other peripheral T-cell lymphomas.The Journal of molecular diagnostics : JMD · 2008Article
- Analysis of T-cell clonality using laser capture microdissection and high-resolution microcapillary electrophoresis.The Journal of molecular diagnostics : JMD · 2007Article
- Protocol for the use of polymerase chain reaction in the detection of intraocular large B-cell lymphoma in ocular samples.The Journal of molecular diagnostics : JMD · 2007Article
- Current status of gastric MALT lymphoma.Current gastroenterology reports · 2006Review
- Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies.Virchows Archiv : an international journal of pathology · 2005Review
- Application of BIOMED-2 primers in fixed and decalcified bone marrow biopsies: analysis of immunoglobulin H receptor rearrangements in B-cell non-Hodgkin's lymphomas.The Journal of molecular diagnostics : JMD · 2005Article
- Detection of API2-MALT1 fusion transcripts in cytologic specimens of patients with pulmonary mucosa-associated lymphoid tissue lymphoma.International journal of hematology · 2005Article
- Review
- Limitations of clonality analysis of B cell proliferations using CDR3 polymerase chain reaction.Molecular pathology : MP · 2000Article
- PCR analysis of the immunoglobulin heavy chain gene in polyclonal processes can yield pseudoclonal bands as an artifact of low B cell number.The Journal of molecular diagnostics : JMD · 2000Article
- Acute lymphoblastic leukaemia: correlation between morphological/immunohistochemical and molecular biological findings in bone marrow biopsy specimens.Molecular pathology : MP · 2000Article
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Authors and funding
4 authors.
Funding
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Abstract
aimsTo evaluate the specificity of standard and fluorescence based (Genescan) polymerase chain reaction (PCR) immunoglobulin heavy chain (IgH) gene rearrangement analysis in complete and microdissected paraffin wax embedded sections from lymphoid proliferations.
methodsPCR IgH gene rearrangement analysis of whole sections and microdissected fragments (n = 62) from paraffin wax embedded reactive lymph nodes (n = 6) and tonsils (n = 3). Amplificant analysis used both standard methods and automated high resolution fluorescence based quantification and size determination using GENESCAN software.
resultsWhole tissue sections were consistently polyclonal in control experiments. IgH gene amplification was successful in 59 of 62 microdissected fragments; only two of 59 showed a polyclonal rearrangement pattern, the remainder being oligoclonal or monoclonal. Reanalysis was possible in 33 samples; six showed reproducible bands on gel analysis and satisfied accepted criteria for monoclonality. Use of high resolution gels with Genescan analysis improved sensitivity and band definition; however, three samples still appeared to be monoclonal.
conclusionsThese results confirm that PCR based IgH gene rearrangement analysis is a sensitive and specific method for demonstrating B cell clonality in whole paraffin wax embedded sections. However, oligoclonal and monoclonal rearrangement patterns are regularly encountered in small tissue fragments from otherwise unremarkable reactive lymphoproliferations, possibly because of preferential priming or detection of local B cell clones. Data from clonal analysis of small, microdissected or lymphocyte poor samples must be evaluated critically. It is recommended that analyses should be run in parallel on at least two tissue specimens. Only reproducible bands present in more than one sample should be considered to be suggestive of neoplasia.
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