ArticleMolecular pathology : MP1999
Expression of the gene encoding the matrix gla protein by mature osteoblasts in human fracture non-unions.
Article in Molecular pathology : MP, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 29 citations in OpenAlex.
- Time to consider fracture nonunion an orphan disease? An update into pathophysiology, epidemiology and therapeutic solutions.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2025Review
- Reduced APPL1 impairs osteogenic differentiation of mesenchymal stem cells by facilitating MGP expression to disrupt the BMP2 pathway in osteoporosis.The Journal of biological chemistry · 2023Article
- Vitamin K Nutrition and Bone Health.Nutrients · 2020Review
- Regulation of Angiogenesis Discriminates Tissue Resident MSCs from Effective and Defective Osteogenic Environments.Journal of clinical medicine · 2020Article
- Biological and molecular profile of fracture non-union tissue: current insights.Journal of cellular and molecular medicine · 2015Review
- Vascular endothelial growth factor improves bone repair in a murine nonunion model.The Iowa orthopaedic journal · 2012Article
- Determination of composition and structure of spongy bone tissue in human head of femur by Raman spectral mapping.Journal of materials science. Materials in medicine · 2011Article
- Recent advances in the use of serological bone formation markers to monitor callus development and fracture healing.Critical reviews in eukaryotic gene expression · 2010Review
- [Osteoblasts : cellular and molecular regulatory mechanisms in fracture healing].Der Orthopade · 2009Article
- Progression of human bone marrow stromal cells into both osteogenic and adipogenic lineages is differentially regulated by structural conformation of collagen I matrix via distinct signaling pathways.Matrix biology : journal of the International Society for Matrix Biology · 2009Article
- Altered relative expression of BMPs and BMP inhibitors in cartilaginous areas of human fractures progressing towards nonunion.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2009Article
- Immunohistochemical localization and mRNA expression of matrix Gla protein and fetuin-A in bone biopsies of hemodialysis patients.Virchows Archiv : an international journal of pathology · 2009Article
- A phenotypic comparison of intervertebral disc and articular cartilage cells in the rat.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2007Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoblast phenotypic abnormality, namely the expression of collagen type III, has been shown previously in fracture non-union woven bone.
aimsTo investigate osteoblasts from fracture non-unions for evidence of gene expression of non-collagenous bone matrix proteins that have been implicated in mineralisation, namely matrix gla protein (MGP), osteonectin, osteopontin, and osteocalcin. MGP is a consistent component of bone matrix, but there are no reports of osteoblasts in the skeleton expressing the gene for MGP, and the site of synthesis of skeletal MGP (perhaps the liver) has yet to be determined.
methodsBiopsies from normally healing human fractures and non-unions were examined by means of in situ hybridisation, using 35S labelled probes and autoradiography to disclose levels of gene expression.
resultsIn normally healing fractures, mature osteoblasts on woven bone were negative for MGP mRNA, but positive for osteonectin, osteopontin, and osteocalcin mRNA molecules. In non-unions, osteoblasts displayed a novel phenotype: they were positive for MGP mRNA, in addition to osteonectin, osteopontin, and osteocalcin mRNA molecules.
conclusionsMature osteoblasts in slowly healing fractures have an unusual phenotype: they express the gene encoding MGP, which indicates that control of osteoblast gene expression in non-unions is likely to be abnormal. This might be of importance in the pathogenesis of non-uniting human fractures, and is of current interest given the emerging status of MGP as an inhibitor of mineralisation.
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