ArticleMolecular pathology : MP1999
Fluorescence in situ hybridisation detection of erbB2 amplification in breast cancer fine needle aspirates.
Article in Molecular pathology : MP, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 26 citations in OpenAlex.
- Monosomy of chromosome 17 in breast cancer during interpretation of HER2 gene amplification.American journal of cancer research · 2015Article
- Monitoring therapeutic response of human ovarian cancer to 17-DMAG by noninvasive PET imaging with (64)Cu-DOTA-trastuzumab.European journal of nuclear medicine and molecular imaging · 2009Article
- Breast cancer and aneusomy 17: implications for carcinogenesis and therapeutic response.The Lancet. Oncology · 2009Review
- Non-invasive PET imaging of EGFR degradation induced by a heat shock protein 90 inhibitor.Molecular imaging and biologyArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a method for the detection of amplification of the erbB2 oncogene in breast cancer fine needle aspirates using fluorescence in situ hybridisation (FISH) and to compare amplification with immunohistochemical detection of the erbB2 protein.
methodsA digoxigenin labelled probe to the erbB2 gene was hybridised to 15 aspirates prepared from operative breast cancer specimens. A chromosome 17 centromere probe was also hybridised to the aspirates either separately or in combination with the erbB2 probe. The aspirates were scored for erbB2 amplification and chromosome 17 centromere number. Subsequently, paraffin wax embedded sections of the tumours were stained with the antibody CB11 and scored for the presence of membrane staining.
resultsThree of the 15 tumour aspirates showed high level amplification of erbB2 detected by FISH. These three tumours also showed chromosome 17 polysomy and diffuse membrane staining by immunohistochemistry.
conclusionsFISH can be used to detect erbB2 amplification in fine needle aspirates and results correlate with conventional immunohistochemical staining. Difficulties were encountered in the visualisation of the signals in non-amplified cases without the use of specialised digital imaging.
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