ArticleMolecular biology of the cell1999
Biparental inheritance of gamma-tubulin during human fertilization: molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 74 citations in OpenAlex.
- Human Sperm Centrosome: From Current Evidence to Future Perspectives-A Systematic Review.Life (Basel, Switzerland) · 2026Review
- The human sperm basal body is a complex centrosome important for embryo preimplantation development.Molecular human reproduction · 2021Article
- Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy.Cells · 2021Article
- Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization.Journal of assisted reproduction and genetics · 2021Article
- The Role of Sperm Centrioles in Human Reproduction - The Known and the Unknown.Frontiers in cell and developmental biology · 2019Review
- Functional Analysis of Human Pathological Semen Samples in an Oocyte Cytoplasmic Ex Vivo System.Scientific reports · 2018Article
- A novel atypical sperm centriole is functional during human fertilization.Nature communications · 2018Article
- Review: Sperm-oocyte interactions and their implications for bull fertility, with emphasis on the ubiquitin-proteasome system.Animal : an international journal of animal bioscience · 2018Review
- Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation.Scientific reports · 2016Article
- Direct Unequal Cleavages: Embryo Developmental Competence, Genetic Constitution and Clinical Outcome.PloS one · 2016Article
- Assisted Reproductive Technologies (ART) with baboons generate live offspring: a nonhuman primate model for ART and reproductive sciences.Reproductive sciences (Thousand Oaks, Calif.) · 2010Article
- Speriolin is a novel human and mouse sperm centrosome protein.Human reproduction (Oxford, England) · 2010Article
- Correlation of human sperm centrosomal proteins with fertility.Journal of human reproductive sciences · 2010Article
- Poor centrosomal function of cat testicular spermatozoa impairs embryo development in vitro after intracytoplasmic sperm injection.Biology of reproduction · 2006Article
- Human sperm centrosomal function during fertilization, a novel assessment for male sterility.Human cell · 2004Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human sperm centrosome reconstitution and the parental contributions to the zygotic centrosome are examined in mammalian zygotes and after exposure of spermatozoa to Xenopus laevis cell-free extracts. The presence and inheritance of the conserved centrosomal constituents gamma-tubulin, centrin, and MPM-2 (which detects phosphorylated epitopes) are traced, as is the sperm microtubule-nucleating capability on reconstituted centrosomes. gamma-Tubulin is biparentally inherited in humans (maternal >> than paternal): Western blots detect the presence of paternal gamma-tubulin. Recruitment of maternal gamma-tubulin to the sperm centrosome occurs after sperm incorporation in vivo or exposure to cell-free extract, especially after sperm "priming" induced by disulfide bond reduction. Centrin is found in the proximal sperm centrosomal region, demonstrates expected calcium sensitivity, but appears absent from the zygotic centrosome after sperm incorporation or exposure to extracts. Sperm centrosome phosphorylation is detected after exposure of primed sperm to egg extracts as well as during the early stages of sperm incorporation after fertilization. Finally, centrosome reconstitution in cell-free extracts permits sperm aster microtubule assembly in vitro. Collectively, these results support a model of a blended zygotic centrosome composed of maternal constituents attracted to an introduced paternal template after insemination.
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