ArticleMolecular biology of the cell1999
Neurite extension occurs in the absence of regulated exocytosis in PC12 subclones.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.Molecular medicine reports · 2015Article
- NGF signaling in PC12 cells: the cooperation of p75(NTR) with TrkA is needed for the activation of both mTORC2 and the PI3K signalling cascade.Biology open · 2013Article
- L1CAM and its cell-surface mutants: new mechanisms and effects relevant to the physiology and pathology of neural cells.Journal of neurochemistry · 2013Article
- Outgrowth of neurites is a dual process.Communicative & integrative biology · 2010Article
- In PC12 cells, expression of neurosecretion and neurite outgrowth are governed by the transcription repressor REST/NRSF.Cellular and molecular neurobiology · 2010Review
- The rest repression of the neurosecretory phenotype is negatively modulated by BHC80, a protein of the BRAF/HDAC complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Identification of a developmentally regulated pathway of membrane retrieval in neuronal growth cones.Journal of cell science · 2008Article
- Involvement of acidic fibroblast growth factor in spinal cord injury repair processes revealed by a proteomics approach.Molecular & cellular proteomics : MCP · 2008Article
- Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery.Molecular biology of the cell · 2006Article
- Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Normal development of embryonic thalamocortical connectivity in the absence of evoked synaptic activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth.The Journal of cell biology · 2000Article
- Neurosecretory cells without neurosecretion: evidence of an independently regulated trait of the cell phenotype.The Journal of physiology · 1999Review
- Comparative studies of PC12 and mouse pheochromocytoma-derived rodent cell lines as models for the study of neuroendocrine systems.In vitro cellular & developmental biology. AnimalArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We have investigated the process leading to differentiation of PC12 cells. This process is known to include extension of neurites and changes in the expression of subsets of proteins involved in cytoskeletal rearrangements or in neurosecretion. To this aim, we have studied a PC12 clone (trk-PC12) stably transfected with the nerve growth factor receptor TrkA. These cells are able to undergo both spontaneous and neurotrophin-induced morphological differentiation. However, both undifferentiated and nerve growth factor-differentiated trk-PC12 cells appear to be completely defective in the expression of proteins of the secretory apparatus, including proteins of synaptic vesicles and large dense-core granules, neurotransmitter transporters, and neurotransmitter-synthesizing enzymes. These results indicate that neurite extension can occur independently of the presence of the neurosecretory machinery, including the proteins that constitute the fusion machine, suggesting the existence of differential activation pathways for the two processes during neuronal differentiation. These findings have been confirmed in independent clones obtained from PC12-27, a previously characterized PC12 variant clone globally incompetent for regulated secretion. In contrast, the integrity of the Rab cycle appears to be necessary for neurite extension, because antisense oligonucleotides against the neurospecific isoform of Rab-guanosine diphosphate-dissociation inhibitor significantly interfere with process formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.