ArticleMolecular biology of the cell1999
Import into and degradation of cytosolic proteins by isolated yeast vacuoles.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Hsp70 chaperones, Ssa1 and Ssa2, limit poly(A) binding protein aggregation.Molecular biology of the cell · 2025Article
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- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- Characterization of the complex formed by β-glucocerebrosidase and the lysosomal integral membrane protein type-2.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Lipid droplet autophagy in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2014Article
- Identification of Ypk1 as a novel selective substrate for nitrogen starvation-triggered proteolysis requiring autophagy system and endosomal sorting complex required for transport (ESCRT) machinery components.The Journal of biological chemistry · 2010Article
- Global analysis of nutrient control of gene expression in Saccharomyces cerevisiae during growth and starvation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Autophagy in yeast: mechanistic insights and physiological function.Microbiology and molecular biology reviews : MMBR · 2001Review
- Cell-free reconstitution of microautophagic vacuole invagination and vesicle formation.The Journal of cell biology · 2000Article
- Autophagic tubes: vacuolar invaginations involved in lateral membrane sorting and inverse vesicle budding.The Journal of cell biology · 2000Article
- The heat shock protein Ssa2p is required for import of fructose-1, 6-bisphosphatase into Vid vesicles.The Journal of cell biology · 2000Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotic cells, both lysosomal and nonlysosomal pathways are involved in degradation of cytosolic proteins. The physiological condition of the cell often determines the degradation pathway of a specific protein. In this article, we show that cytosolic proteins can be taken up and degraded by isolated Saccharomyces cerevisiae vacuoles. After starvation of the cells, protein uptake increases. Uptake and degradation are temperature dependent and show biphasic kinetics. Vacuolar protein import is dependent on cytosolic heat shock proteins of the hsp70 family and on protease-sensitive component(s) on the outer surface of vacuoles. Degradation of the imported cytosolic proteins depends on a functional vacuolar ATPase. We show that the cytosolic isoform of yeast glyceraldehyde-3-phosphate dehydrogenase is degraded via this pathway. This import and degradation pathway is reminiscent of the protein transport pathway from the cytosol to lysosomes of mammalian cells.
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