ArticleApplied and environmental microbiology1999
A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Protozoan Parasites in Drinking Water: A System Approach for Improved Water, Sanitation and Hygiene in Developing Countries.International journal of environmental research and public health · 2018Review
- In vitro inhibitory effects of plant-derived by-products against Cryptosporidium parvum.Parasite (Paris, France) · 2016Article
- Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems.BMC microbiology · 2014Article
- Cryptosporidium and Giardia in surface water: a case study from Michigan, USA to inform management of rural water systems.International journal of environmental research and public health · 2014Article
- In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds.Parasitology research · 2012Article
- Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.Applied and environmental microbiology · 2012Article
- Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.Applied and environmental microbiology · 2010Article
- Cryptosporidium parvum isolate-dependent postinfectious jejunal hypersensitivity and mast cell accumulation in an immunocompetent rat model.Infection and immunity · 2009Article
- Interactions of Cryptosporidium parvum, Giardia lamblia, vaccinal poliovirus type 1, and bacteriophages phiX174 and MS2 with a drinking water biofilm and a wastewater biofilm.Applied and environmental microbiology · 2008Article
- Aged HCT-8 cell monolayers support Cryptosporidium parvum infection.Applied and environmental microbiology · 2007Article
- Impact of zooplankton grazing on the excystation, viability, and infectivity of the protozoan pathogens Cryptosporidium parvum and Giardia lamblia.Applied and environmental microbiology · 2007Article
- Use of cell culture to assess Cryptosporidium parvum survival rates in natural groundwaters and surface waters.Applied and environmental microbiology · 2007Article
- Detection of infectious Cryptosporidium oocysts by cell culture immunofluorescence assay: applicability to environmental samples.Applied and environmental microbiology · 2005Article
- Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection.Applied and environmental microbiology · 2005Article
- Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.Applied and environmental microbiology · 2005Article
- Effects of the Norwegian winter environment on Giardia cysts and Cryptosporidium oocysts.Microbial ecology · 2004Article
- Assessment of methods for detection of infectious Cryptosporidium oocysts and Giardia cysts in reclaimed effluents.Applied and environmental microbiology · 2003Article
- Infectious Cryptosporidium parvum oocysts in final reclaimed effluent.Applied and environmental microbiology · 2003Article
- Comparison of method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters.Applied and environmental microbiology · 2003Article
- Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium is globally established as a contaminant of drinking and recreational waters. A previously described cell culture infectivity assay capable of detecting infectious oocysts was adapted to quantify viable oocysts through sporozoite invasion and clustering of foci. Eight experiments were performed by using oocysts less than 4 months of age to inoculate host HCT-8 cell monolayers. Oocysts were diluted in a standard 5- or 10-fold multiple dilution format, levels of infection and clustering were determined, and the most probable number (MPN) of infectious oocysts in the stock suspension was calculated. The MPN was compared to the initial oocyst inoculum to determine the level of correlation. For oocysts less than 30 days of age, the correlation coefficient (r) was 0.9726 (0.9306 to 0.9893; n = 20). A two-tailed P value (alpha = 0.05) indicated that P was less than 0.0001. This strong correlation suggests that the MPN can be used to effectively enumerate infectious oocysts in a cell culture system. Age affected the degree of oocyst infectivity. Oocyst infectivity was tested by the focus detection method (FDM)-MPN assay and in BALB/c mice before and after treatment with pulsed white light (PureBrite). The FDM-MPN assay and animal infectivity assays both demonstrated more than a 4 log(10) inactivation. Municipal water systems and a host of other water testing organizations could utilize the FDM-MPN assay for routine survival and disinfection studies.
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