ArticleGenetics1999
Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
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- An evaluation of ChatGPT and Bard (Gemini) in the context of biological knowledge retrieval.Access microbiology · 2024Article
- Article
- mRNA Turnover Protein 4 Is Vital for Fungal Pathogenicity and Response to Oxidative Stress inPathogens (Basel, Switzerland) · 2023Article
- Recombinant expression and biophysical characterization of Mrt4 protein that involved in mRNA turnover and ribosome assembly fromBioengineered · 2022Article
- Investigation the global effect of rare earth gadolinium on the buddingFrontiers in microbiology · 2022Article
- MoYvh1 subverts rice defense through functions of ribosomal protein MoMrt4 in Magnaporthe oryzae.PLoS pathogens · 2018Article
- Placeholder factors in ribosome biogenesis: please, pave my way.Microbial cell (Graz, Austria) · 2017Review
- Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis.The EMBO journal · 2017Article
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit.Nature structural & molecular biology · 2016Article
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi.Protein & cell · 2011Article
- Yvh1 is required for a late maturation step in the 60S biogenesis pathway.The Journal of cell biology · 2009Article
- Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0.The Journal of cell biology · 2009Article
- The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein.Nucleic acids research · 2009Article
- Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit.Nucleic acids research · 2008Article
- Article
- Rck2 is required for reprogramming of ribosomes during oxidative stress.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In a screen for factors involved in mRNA turnover, four temperature-sensitive yeast strains (ts1189, ts942, ts817, and ts1100) exhibited defects in the decay of several mRNAs. Complementation of the growth and mRNA decay defects, and genetic experiments, revealed that ts1189 is mutated in the previously unknown MRT4 gene, ts942 is mutated in GRC5 (encoding the L9 ribosomal protein), ts817 contains a mutation in SLA2 (encoding a membrane protein), and ts1100 contains a mutation in THS1 (encoding the threonyl-tRNA synthetase). Three of the four mutants (mrt4, grc5, and sla2) were not defective in protein synthesis, suggesting that these strains contain mutations in factors that may play a specific role in mRNA decay. The mRNA stabilization observed in the ths1 strain, however, could be due to the significant drop in translation observed in this mutant at 37 degrees. While the three interesting mutants appear to encode novel mRNA decay factors, at least one could be linked to a previously characterized mRNA decay pathway. The growth and mRNA decay defects of ts942 (grc5) cells were suppressed by overexpression of the NMD3 gene, encoding a protein shown to participate in a two-hybrid interaction with the nonsense-mediated decay protein Upf1p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.