ArticleGenetics1999
Mutational adaptation of Escherichia coli to glucose limitation involves distinct evolutionary pathways in aerobic and oxygen-limited environments.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Adaptive transcriptional remodeling ofNpj complexity · 2026Article
- Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation.BMC biology · 2025Article
- Evolutionary dynamics and structural consequences of de novo beneficial mutations and mutant lineages arising in a constant environment.BMC biology · 2021Article
- A shifting mutational landscape in 6 nutritional states: Stress-induced mutagenesis as a series of distinct stress input-mutation output relationships.PLoS biology · 2017Article
- Dynamics and genetic diversification ofPeerJ · 2017Article
- The functional basis of adaptive evolution in chemostats.FEMS microbiology reviews · 2015Review
- Adaptive laboratory evolution -- principles and applications for biotechnology.Microbial cell factories · 2013Review
- Glucose transport in Escherichia coli mutant strains with defects in sugar transport systems.Journal of bacteriology · 2012Article
- Stock culture heterogeneity rather than new mutational variation complicates short-term cell physiology studies of Escherichia coli K-12 MG1655 in continuous culture.Microbiology (Reading, England) · 2011Article
- Adaptation in a mouse colony monoassociated with Escherichia coli K-12 for more than 1,000 days.Applied and environmental microbiology · 2010Article
- E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.PLoS genetics · 2009Article
- Fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation.PLoS genetics · 2009Article
- The influence of cellular physiology on the initiation of mutational pathways in Escherichia coli populations.Proceedings. Biological sciences · 2003Article
- Widespread N-acetyl-D-glucosamine uptake among pelagic marine bacteria and its ecological implications.Applied and environmental microbiology · 2002Article
- Article
- Regulation of mutY and nature of mutator mutations in Escherichia coli populations under nutrient limitation.Journal of bacteriology · 2002Article
- Speed versus efficiency in microbial growth and the role of parallel pathways.Journal of bacteriology · 2002Article
- Experimental analysis of molecular events during mutational periodic selections in bacterial evolution.Genetics · 2000Article
- Substrate specificity and signal transduction pathways in the glucose-specific enzyme II (EII(Glc)) component of the Escherichia coli phosphotransferase system.Journal of bacteriology · 2000Article
- Facilitated diffusion of fructose via the phosphoenolpyruvate/glucose phosphotransferase system of Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutational adaptations leading to improved glucose transport were followed with Escherichia coli K-12 growing in glucose-limited continuous cultures. When populations were oxygen limited as well as glucose limited, all bacteria within 280 generations contained mutations in a single codon of the ptsG gene. V12F and V12G replacements in the enzyme IIBC(Glc) component of the glucose phosphotransferase system were responsible for improved transport. In stark contrast, ptsG mutations were uncommon in fully aerobic glucose-limited cultures, in which polygenic mutations in mgl, mlc, and malT (regulating an alternate high-affinity Mgl/LamB uptake pathway) spread through the adapted population. Hence the same organism adapted to the same selection (glucose limitation) by different evolutionary pathways depending on a secondary environmental factor. The clonal diversity in the adapted populations was also significantly different. The PtsG V12F substitution under O(2) limitation contributed to a universal "winner clone" whereas polygenic, multiallelic changes led to considerable polymorphism in aerobic cultures. Why the difference in adaptive outcomes? E. coli physiology prevented scavenging by the LamB/Mgl system under O(2) limitation; hence, ptsG mutations provided the only adaptive pathway. But ptsG mutations in aerobic cultures are overtaken by mgl, mlc, and malT adaptations with better glucose-scavenging ability. Indeed, when an mglA::Tn10 mutant with an inactivated Mgl/LamB pathway was introduced into two independent aerobic chemostats, adaptation of the Mgl(-) strain involved the identical ptsG mutation found under O(2)-limited conditions with wild-type or Mgl(-) bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.