ArticleAntimicrobial agents and chemotherapy1999
Comparative studies of mutations in animal isolates and experimental in vitro- and in vivo-selected mutants of Salmonella spp. suggest a counterselection of highly fluoroquinolone-resistant strains in the field.
Article in Antimicrobial agents and chemotherapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed.
- Mechanism-Base Pharmacokinetic-Pharmacodynamic Modeling of Cefquinome AgainstMedical sciences (Basel, Switzerland) · 2026Article
- Antimicrobial resistance surveillance and focused comparative genomics of clinicalMicrobiology spectrum · 2026Article
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- Genomic analyses of drug-resistant Salmonella enterica serovar Heidelberg strains isolated from meat and related sources between 2013 and 2017 in the south region of Brazil.Current genetics · 2023Article
- Role of the Two-Component System CiaRH in the Regulation of Efflux Pump SatAB and Its Correlation with Fluoroquinolone Susceptibility.Microbiology spectrum · 2022Article
- Surveillance of antimicrobial-resistant Escherichia coli in Sheltered dogs in the Kanto Region of Japan.Scientific reports · 2022Article
- The molecular mechanisms of fluoroquinolone resistance found in rectal swab isolates of Enterobacterales from men undergoing a transrectal prostate biopsy: the rationale for targeted prophylaxis.Annals of clinical microbiology and antimicrobials · 2021Article
- A Single Nucleotide Polymorphism inFrontiers in microbiology · 2021Article
- Discovery of seven novel mutations of gyrB, parC and parE in Salmonella Typhi and Paratyphi strains from Jiangsu Province of China.Scientific reports · 2020Article
- Salmonella enterica subsp.Applied and environmental microbiology · 2019Article
- Association ofFood safety (Tokyo, Japan) · 2018Article
- The effects of different enrofloxacin dosages on clinical efficacy and resistance development in chickens experimentally infected with Salmonella Typhimurium.Scientific reports · 2017Article
- Using In Vitro Dynamic Models To Evaluate Fluoroquinolone Activity against Emergence of Resistant Salmonella enterica Serovar Typhimurium.Antimicrobial agents and chemotherapy · 2017Article
- The PK-PD Relationship and Resistance Development of Danofloxacin againstFrontiers in microbiology · 2017Article
- Antimicrobial Resistance ofFrontiers in microbiology · 2017Article
- Multidrug-Resistant Shigella Infections in Patients with Diarrhea, Cambodia, 2014-2015.Emerging infectious diseases · 2016Article
- Molecular epidemiology and characterization of multiple drug-resistant (MDR) clinical isolates of Acinetobacter baumannii.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2015Article
- Enteric fever imported to the Czech Republic: epidemiology, clinical characteristics and antimicrobial susceptibility.Folia microbiologica · 2015Article
- A novel technique for detecting antibiotic-resistant typhoid from rapid diagnostic tests.Journal of clinical microbiology · 2015Article
17 more citing papers are in PubMed but not listed here.
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Abstract
The occurrence of mutations in the genes coding for gyrase (gyrA and gyrB) and topoisomerase IV (parE and parC) of Salmonella typhimurium experimental mutants selected in vitro and in vivo and of 138 nalidixic acid-resistant Salmonella field isolates was investigated. The sequencing of the quinolone resistance-determining region of these genes in highly fluoroquinolone-resistant mutants (MICs of 4 to 16 microg/ml) revealed the presence of gyrA mutations at codons corresponding to Gly-81 or Ser-83, some of which were associated with a mutation at Asp-87. No mutations were found in the gyrB, parC, and parE genes. An assay combining allele-specific PCR and restriction fragment length polymorphism was developed to rapidly screen mutations at codons 81, 83, and 87 of gyrA. The MICs of ciprofloxacin for the field isolates reached only 2 microg/ml, versus 16 microg/ml for some in vitro-selected mutants. The field isolates, like the mutants selected in vivo, had only a single gyrA mutation at codon 83 or 87. Single gyrA mutations were also found in highly resistant in vitro-selected mutants (MIC of ciprofloxacin, 8 microg/ml), which indicates that mechanisms other than the unique modification of the intracellular targets could participate in fluoroquinolone resistance in Salmonella spp. A comparison of experimental mutants selected in vitro, field strains, and mutants selected in vivo suggests that highly fluoroquinolone-resistant strains are counterselected in field conditions in the absence of selective pressure.
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