ArticleBritish journal of cancer1999
Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow.
Article in British journal of cancer, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Colon Cancer Diagnosis Based on Machine Learning and Deep Learning: Modalities and Analysis Techniques.Sensors (Basel, Switzerland) · 2022Review
- Circulatory shear stress induces molecular changes and side population enrichment in primary tumor-derived lung cancer cells with higher metastatic potential.Scientific reports · 2021Article
- Article
- The role of toll-like receptors in colorectal cancer progression: evidence for epithelial to leucocytic transition.Frontiers in immunology · 2014Review
- Hsa-miR-574-5p negatively regulates MACC-1 expression to suppress colorectal cancer liver metastasis.Cancer cell international · 2014Article
- Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding.The Journal of biological chemistry · 2012Article
- Shear flow-induced formation of tubular cell protrusions in multiple myeloma cells.Journal of cellular physiology · 2011Article
- Interactions between colon cancer cells and hepatocytes in rats in relation to metastasis.Journal of cellular and molecular medicine · 2008Article
- Integrin alpha v beta 6 mediates the potential for colon cancer cells to colonize in and metastasize to the liver.Cancer science · 2008Article
- Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential.The American journal of pathology · 2006Article
- Shear stress induced release of von Willebrand factor and thrombospondin-1 in HUVEC extracellular matrix enhances breast tumour cell adhesion.Clinical & experimental metastasis · 2005Article
- The laminin receptor modulates granulocyte-macrophage colony-stimulating factor receptor complex formation and modulates its signaling.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mechanical properties of hepatocellular carcinoma cells.World journal of gastroenterology · 2002Article
- An intravital model to monitor steps of metastatic tumor cell adhesion within the hepatic microcirculation.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary TractArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Under the physiological shear condition, cultured colon cancer cells bound to laminin (LM), but not to fibronectin or vitronectin. Most of the tethered cells did not roll, but arrested immediately and spread within 10-30 min on LM under the continuous presence of shear flow. The tethering of Colo201 was partially inhibited by monoclonal antibodies (mAbs) to alpha6 integrin and a combination of mAbs to beta1 and beta4 integrins, but not by mAb to 67KD laminin receptor. Some Colo201 cells still tethered at 4 degrees C. This suggests that alpha6beta1 and alpha6beta4 integrins participate in Colo201 tethering on LM, although other non-integrin molecules play roles. In contrast, the spread of Colo201 was effectively inhibited by the mAbs to integrin alpha2, alpha6 and beta1 chains. The effect of anti-alpha2 plus anti-alpha6 mAbs was almost equal to anti-beta1, suggesting that Colo201 cells mainly use alpha2beta1 and alpha6beta1 integrins for spreading on LM. When the cells were perfused on subconfluent endothelial cells (HUVEC) cultured on LM, they did not tether on HUVEC but did on coated LM exposed at intercellular gap area. Immunohistochemistry revealed that LM abundantly existed in the cytosol of human portal and hepatic vein endothelial cells. These data suggest that LM can mediate from tethering to spreading of colon cancer cells under the blood flow and plays an essential role in haematogeneous metastasis.
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