ArticleProceedings of the National Academy of Sciences of the United States of America1999
Syntaxin 1 interacts with the L(D) subtype of voltage-gated Ca(2+) channels in pancreatic beta cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 138 citations in OpenAlex.
- Non-ionotropic voltage-gated calcium channel signaling.Channels (Austin, Tex.) · 2024Review
- Stx4 is required to regulate cardiomyocyte CaHGG advances · 2022Article
- Beta-Cell Adaptation to Pregnancy - Role of Calcium Dynamics.Frontiers in endocrinology · 2022Review
- Enhanced expression of β cell CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The eye as a novel imaging site in diabetes research.Pharmacology & therapeutics · 2019Review
- Diet-induced β-cell insulin resistance results in reversible loss of functional β-cell mass.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Duodeno-jejunal bypass restores β-cell hypersecretion and islet hypertrophy in western diet obese rats.Endocrine · 2018Article
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- SNAP-25b-deficiency increases insulin secretion and changes spatiotemporal profile of CaScientific reports · 2017Article
- Syntaxin-3 Binds and Regulates Both R- and L-Type Calcium Channels in Insulin-Secreting INS-1 832/13 Cells.PloS one · 2016Article
- CaV1.2 and CaV1.3 channel hyperactivation in mouse islet β cells exposed to type 1 diabetic serum.Cellular and molecular life sciences : CMLS · 2015Article
- Ionic mechanisms in pancreatic β cell signaling.Cellular and molecular life sciences : CMLS · 2014Review
- The voltage-dependent potassium channel subunit Kv2.1 regulates insulin secretion from rodent and human islets independently of its electrical function.Diabetologia · 2012Article
- Gelsolin associates with the N terminus of syntaxin 4 to regulate insulin granule exocytosis.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Pancreatic function, type 2 diabetes, and metabolism in aging.International journal of endocrinology · 2012Article
- Glucose-dependent docking and SNARE protein-mediated exocytosis in mouse pancreatic alpha-cell.Pflugers Archiv : European journal of physiology · 2011Article
- Inositol 1, 4, 5-trisphosphate receptor interacts with the SNARE domain of syntaxin 1B.The journal of physiological sciences : JPS · 2011Article
- Article
- Subthreshold α₂-adrenergic activation counteracts glucagon-like peptide-1 potentiation of glucose-stimulated insulin secretion.Experimental diabetes research · 2011Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interaction of syntaxin 1 with the alpha(1D) subunit of the voltage-gated L type Ca(2+) channel was investigated in the pancreatic beta cell. Coexpression of the enhanced green fluorescent protein-linked alpha(1D) subunit with the enhanced blue fluorescent protein-linked syntaxin 1 and Western blot analysis together with subcellular fractionation demonstrated that the alpha(1D) subunit and syntaxin 1 were colocalized in the plasma membrane. Furthermore, the alpha(1D) subunit was coimmunoprecipitated efficiently by a polyclonal antibody against syntaxin 1. Syntaxin 1 also played a central role in the modulation of L type Ca(2+) channel activity because there was a faster Ca(2+) current run-down in cells incubated with antisyntaxin 1 compared with controls. In parallel, antisyntaxin 1 markedly reduced insulin release in both intact and permeabilized cells, subsequent to depolarization with K(+) or exposure to high Ca(2+). Exchanging Ca(2+) for Ba(2+) abolished the effect of antisyntaxin 1 on both Ca(2+) channel activity and insulin exocytosis. Moreover, antisyntaxin 1 had no significant effects on Ca(2+)-independent insulin release trigged by hypertonic stimulation. This suggests that there is a structure-function relationship between the alpha(1D) subunit of the L type Ca(2+) channel and the exocytotic machinery in the pancreatic beta cell.
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