ArticleProceedings of the National Academy of Sciences of the United States of America1999
The beta-globin promoter is important for recruitment of erythroid Krüppel-like factor to the locus control region in erythroid cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- The Molecular Pathology of Non-Malignant Haematological Disease.British journal of biomedical science · 2026Review
- Interleukin-12: an update on its immunological activities, signaling and regulation of gene expression.Current immunology reviews · 2005Article
- Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells.Nucleic acids research · 2005Review
- Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages.The Journal of biological chemistry · 2004Article
- Context-dependent regulation of GATA-1 by friend of GATA-1.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.Nucleic acids research · 2003Article
- Beta-globin locus control region HS2 and HS3 interact structurally and functionally.Nucleic acids research · 2003Article
- Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Unanticipated repression function linked to erythroid Krüppel-like factor.Molecular and cellular biology · 2001Article
- Role of NF-Y in in vivo regulation of the gamma-globin gene.Molecular and cellular biology · 2001Article
- Reconstitution of human beta-globin locus control region hypersensitive sites in the absence of chromatin assembly.Molecular and cellular biology · 2001Article
- The regulatory network controlling beta-globin gene switching.Molecular biology reports · 2001Review
- The role of trans-acting factors and DNA-bending in the silencing of human beta-globin gene expression.Nucleic acids research · 2000Article
- Erythroid Kruppel-like factor is recruited to the CACCC box in the beta-globin promoter but not to the CACCC box in the gamma-globin promoter: the role of the neighboring promoter elements.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Targeting a SWI/SNF-related chromatin remodeling complex to the beta-globin promoter in erythroid cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erythroid Krüppel-like factor (EKLF), which binds to the CACCC box in the beta-globin promoter, is required for the expression of the beta-globin gene in adult erythroid cells. It was recently demonstrated that EKLF is also required for the activity of the beta-globin locus control region (LCR) 5'HS3. Some evidence suggests that the LCR and the beta-globin promoter interact in adult erythroid cells, and the network of protein-protein interactions that exists between these two elements may regulate how EKLF is recruited to the LCR. In this report, we use the PIN*POINT assay to study the role of the promoter on the recruitment of EKLF to 5'HS2 and 5'HS3 of the LCR. We find that recruitment of EKLF to 5'HS2 requires the TATA box, but recruitment to 5'HS3 depends on the CACCC and TATA boxes of the beta-globin promoter. Furthermore, recruitment of EKLF to 5'HS3 only occurred in beta-globin-expressing murine erythroid leukemia cells, whereas recruitment of EKLF to 5'HS2 occurred in both gamma-globin-expressing K562 cells and murine erythroid leukemia cells. Unlike EKLF, Sp1, which also binds to CACCC boxes, is not recruited to 5'HS3. We have also examined how one 5'HS affects the recruitment of EKLF to another 5'HS. We have found that the recruitment of EKLF to 5'HS3 depends on the presence of 5'HS2 in cis, but the recruitment to 5'HS2 does not depend on 5'HS3. Based on these results, we present a model that illustrates how EKLF may be recruited to the beta-globin locus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.