ReviewPharmaceutical research1999
Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research.
Review in Pharmaceutical research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 199 citations in OpenAlex.
- Tunable acoustic rotation for deep biophysical phenotyping of preclinical Alzheimer's disease.Materials today. Bio · 2026Article
- Spatially and Chemically Specific Optical Control of Cells via Supervised and Automated Target Selection.ACS photonics · 2025Article
- Highly sensitive AIEE active fluorescent probe for detection of deferasirox: extensive experimental and theoretical studies.RSC advances · 2024Article
- Milk exosomes anchored with hydrophilic and zwitterionic motifs enhance mucus permeability for applications in oral gene delivery.Biomaterials science · 2024Article
- Article
- Postsynaptic protein assembly in three and two dimensions studied by mesoscopic simulations.Biophysical journal · 2023Article
- Study liquid-liquid phase separation with optical microscopy: A methodology review.APL bioengineering · 2023Review
- Measurement of Protein and Nucleic Acid Diffusion Coefficients Within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics.PLoS computational biology · 2022Article
- Article
- Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion.Journal of mathematical biology · 2021Article
- Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- A Life Cycle for Modeling Biology at Different Scales.Frontiers in plant science · 2021Review
- Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.Biophysical journal · 2020Article
- Improved FRAP Measurements on Biofilms.Biophysical journal · 2020Article
- Isochronal superpositioning in the equilibrium regime of superpressed propylene carbonate to ∼ 1.8 GPa: A study by diffusivity measurement of the fluorescent probe Coumarin 1.The European physical journal. E, Soft matter · 2019Article
- VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.Optics express · 2019Article
- Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains.The FEBS journal · 2019Review
- Single-Emulsion P(HB-HV) Microsphere Preparation Tuned by Copolymer Molar Mass and Additive Interaction.ACS omega · 2019Article
- Layer by Layer Mesoporous Silica-Hyaluronic Acid-Cyclodextrin Bifunctional "Lamination": Study of the Application of Fluorescent Probe and Host⁻Guest Interactions in the Drug Delivery Field.Materials (Basel, Switzerland) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This review introduces the basics of fluorescence recovery after photobleaching (FRAP) from a theoretical and an instrumentational approach. The most interesting and innovative applications with a pharmaceutical point of view are briefly discussed and possible future applications are suggested. These future applications include research on the mobility of macromolecular drugs in macro- or microscopic pharmaceutical dosage forms, mobility, and binding of antitumor drugs in tumor tissue, intracellular trafficking of gene complexes and mobility of drugs in membranes prior to transmembrane penetration. The paper is also intended to be an introductory guideline to those who would like to get involved in FRAP related experimental techniques. Therefore, comprehensive details on different setups and data analysis are given, as well as a brief outline of the problems that may be encountered when performing FRAP. Overall, this review shows the great potential of FRAP in pharmaceutical research. This is complemented by our own results illustrating the possibility of performing FRAP in microscopic dosage forms (microspheres) using a high resolution variant of FRAP.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.