ArticleOncogene1999
Transcriptional regulation by targeted recruitment of cyclin-dependent CDK9 kinase in vivo.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting.Nature communications · 2021Article
- Aminopyrazole based CDK9 PROTAC sensitizes pancreatic cancer cells to venetoclax.Bioorganic & medicinal chemistry letters · 2021Article
- Differences in Transcriptional Dynamics Between T-cells and Macrophages as Determined by a Three-State Mathematical Model.Scientific reports · 2020Article
- Different modes of enhancer-specific regulation by Runt and Even-skipped duringMolecular biology of the cell · 2017Article
- The cellular bromodomain protein Brd4 has multiple functions in E2-mediated papillomavirus transcription activation.Viruses · 2014Article
- Role of noncoding RNAs in the regulation of P-TEFb availability and enzymatic activity.BioMed research international · 2014Review
- Transcription elongation regulator 1 (TCERG1) regulates competent RNA polymerase II-mediated elongation of HIV-1 transcription and facilitates efficient viral replication.Retrovirology · 2013Article
- MYCN-mediated transcriptional repression in neuroblastoma: the other side of the coin.Frontiers in oncology · 2013Article
- Defining the status of RNA polymerase at promoters.Cell reports · 2012Article
- HIV-1 replication and latency are regulated by translational control of cyclin T1.Journal of molecular biology · 2011Article
- Recruitment of cdk9 to the immediate-early viral transcriptosomes during human cytomegalovirus infection requires efficient binding to cyclin T1, a threshold level of IE2 86, and active transcription.Journal of virology · 2009Article
- Characterizing complex dynamics in the transactivation response element apical loop and motional correlations with the bulge by NMR, molecular dynamics, and mutagenesis.Biophysical journal · 2008Article
- P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo.Molecular and cellular biology · 2008Article
- Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation.Molecular and cellular biology · 2008Article
- Article
- Regulation of P-TEFb elongation complex activity by CDK9 acetylation.Molecular and cellular biology · 2007Article
- Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription.Journal of molecular biology · 2007Article
- Effect of SWI/SNF chromatin remodeling complex on HIV-1 Tat activated transcription.Retrovirology · 2006Article
- Inhibition of Tat activity by the HEXIM1 protein.Retrovirology · 2005Article
- HIV-1 Tat stimulates transcription complex assembly through recruitment of TBP in the absence of TAFs.PLoS biology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CDK9 kinase in association with Cyclin T is a component of the transcription positive-acting complex pTEFb which facilitates the transition from abortive to productive transcription elongation by phosphorylating the carboxyl-terminal domain of RNA polymerase II. The Cyclin T1/CDK9 complex is implicated in Tat transactivation, and it has been suggested that Tat functions by recruiting this complex to RNAPII through cooperative binding to RNA. Here, we demonstrate that targeted recruitment of Cyclin T1/CDK9 kinase complex to specific promoters, through fusion to a DNA-binding domain of either Cyclin T1 or CDK9 kinase, stimulates transcription in vivo. Transcriptional enhancement was dependent on active CDK9, as a catalytically inactive form had no transcriptional effect. We determined that, unlike conventional activators, DNA-bound CDK9 does not activate enhancerless TATA-promoters unless TBP is overexpressed, suggesting that CDK9 acts in vivo at a step subsequent to TFIID recruitment DNA-bound. Finally, we determined that CDK9-mediated transcriptional activation is mediated by preferentially stimulating productive transcription elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.