ArticleJournal of bacteriology1999
Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 130 citations in OpenAlex.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- A Copper-Inducible Regulatory System for Dynamic Enhancement of Gene Editing Efficiency in Yarrowia lipolytica.Biotechnology journal · 2026Article
- Article
- Evaluation of Shifts of Gene Transcription Levels of Unicellular Green AlgaMicroorganisms · 2023Article
- Biosynthesis of insect sex pheromone precursors via engineered β-oxidation in yeast.FEMS yeast research · 2022Article
- Characterization of Acyl-CoA Oxidases from the Lipolytic YeastJournal of microbiology and biotechnology · 2022Article
- Multiple acyl-CoA dehydrogenase deficiency kills Mycobacterium tuberculosis in vitro and during infection.Nature communications · 2021Article
- Integrating transcriptomic and metabolomic analysis of the oleaginous yeast Rhodosporidium toruloides IFO0880 during growth under different carbon sources.Applied microbiology and biotechnology · 2021Article
- Fermentation process for producing CFAs using Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2020Article
- Genetic Manipulation of a Lipolytic YeastMicroorganisms · 2020Article
- Cellular and metabolic engineering of oleaginous yeastEngineering in life sciences · 2019Review
- Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones.Microbial cell factories · 2019Article
- Development of a promising microbial platform for the production of dicarboxylic acids from biorenewable resources.Biotechnology for biofuels · 2018Article
- New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.Microbial cell factories · 2017Article
- A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica.Applied microbiology and biotechnology · 2017Article
- Integrating Cellular and Bioprocess Engineering in the Non-Conventional YeastFrontiers in bioengineering and biotechnology · 2017Review
- Biotechnological production of γ-decalactone, a peach like aroma, by Yarrowia lipolytica.World journal of microbiology & biotechnology · 2016Review
- Regulation of amino-acid metabolism controls flux to lipid accumulation inNPJ systems biology and applications · 2016Article
- Engineering the Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel.PloS one · 2014Article
- A genome-scale metabolic model of the lipid-accumulating yeast Yarrowia lipolytica.BMC systems biology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified five acyl coenzyme A (CoA) oxidase isozymes (Aox1 through Aox5) in the n-alkane-assimilating yeast Yarrowia lipolytica, encoded by the POX1 through POX5 genes. The physiological function of these oxidases has been investigated by gene disruption. Single, double, triple, and quadruple disruptants were constructed. Global Aox activity was determined as a function of time after induction and of substrate chain length. Single null mutations did not affect growth but affected the chain length preference of acyl-CoA oxidase activity, as evidenced by a chain length specificity for Aox2 and Aox3. Aox2 was shown to be a long-chain acyl-CoA oxidase and Aox3 was found to be active against short-chain fatty acids, whereas Aox5 was active against molecules of all chain lengths. Mutations in Aox4 and Aox5 resulted in an increase in total Aox activity. The growth of mutant strains was analyzed. In the presence of POX1 only, strains did not grow on fatty acids, whereas POX4 alone elicited partial growth, and the growth of the double POX2-POX3-deleted mutant was normal excepted on plates containing oleic acid as the carbon source. The amounts of Aox protein detected by Western blotting paralleled the Aox activity levels, demonstrating the regulation of Aox in cells according to the POX genotype.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.