ArticleThe British journal of ophthalmology1999
Culture and characterisation of epithelial cells from human pterygia.
Article in The British journal of ophthalmology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Evaluation of antimitotic and antiangiogenic effect of preoperative subconjunctival application of mitomycin C in primary pterygium: a randomized trial.International ophthalmology · 2019Trial
- Autophagy-related pathway dysregulation and apoptosis suppression in pterygium: Role of key biomarkers Beclin-1, ATG5, LC3, p62, and Bcl-2 in pathogenesis and potential nonsurgical targets.The Journal of international medical research · 2026Observational
- Rapid 3D bioprinting of a multicellular model recapitulating pterygium microenvironment.Biomaterials · 2022Article
- Is the Conjunctiva a Potential Target for Advanced Therapy Medicinal Products?Pharmaceutics · 2021Review
- TheInternational journal of medical sciences · 2020Article
- Corneal Reinnervation and Sensitivity Recovery after Pterygium Excision.Journal of ophthalmology · 2020Article
- Article
- MicroRNA-215 Regulates Fibroblast Function: Insights from a Human Fibrotic Disease.Cell cycle (Georgetown, Tex.) · 2015Article
- Effect of porcine chondrocyte-derived extracellular matrix on the pterygium in mouse model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2014Article
- Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells.Molecular vision · 2009Article
- Calcium-binding S100 protein expression in pterygium.Molecular vision · 2009Article
- Localization of the low-affinity nerve growth factor receptor p75 in human limbal epithelial cells.Journal of cellular and molecular medicine · 2008Article
- Cultured human ocular surface epithelium on therapeutic contact lenses.The British journal of ophthalmology · 2007Article
- The role of ultraviolet irradiation and heparin-binding epidermal growth factor-like growth factor in the pathogenesis of pterygium.The American journal of pathology · 2003Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimsPterygia are a common disorder of the ocular surface. The disease represents a chronic fibrovascular and degenerative process thought to originate at the conjunctival-corneal junction, where altered limbal stem cells are proposed to be the cell of origin. Extensive epidemiological evidence exists to implicate ultraviolet B irradiation in the pathogenesis of pterygia. To date no animal or in vitro culture model has been developed to test such an hypothesis. The aim of this study was to establish and characterise a pure population of epithelial cells derived from pterygium tissue.
methodsTissue specimens were obtained from patients undergoing pterygium excision. Explants were cultured in either serum free or serum supplemented medium. Primary and passaged cells were processed for light microscopy, analysed by flow cytometry, and characterised immunohistochemically using specific antibodies.
resultsIn serum free culture, cuboidal cells with typical morphology of epithelial cells migrated from the pterygium explants from 3 days onwards and eventually formed a cohesive monolayer. Passaged cells consisted of 98.4% cytokeratin positive cells and demonstrated immunoreactivity for multiple cytokeratins, including AE1, AE3, AE5, but were negative for AE8. These cells also expressed an epithelial specific antigen, together with vimentin and mucin, as did epithelial cells in sections of pterygia.
conclusionsA relatively simple method of isolating pterygium epithelial cells has been established. Cultured pterygium epithelial cells are phenotypically and functionally similar to their in vivo counterparts with respect to keratin, vimentin, and mucin expression. In vitro assays using these cells may aid in elucidating the pathogenesis of pterygia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.