ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus Laevis oocytes.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 271 citations in OpenAlex.
- Extra-nasal odorant receptors: molecular mechanisms and therapeutic implications.Frontiers in physiology · 2026Review
- M2OR: a database of olfactory receptor-odorant pairs for understanding the molecular mechanisms of olfaction.Nucleic acids research · 2024Article
- Olfactory Receptors as an Emerging Chemical Sensing Scaffold.Biochemistry · 2023Review
- Transcuticular calcium imaging as a tool for the functional study of insect odorant receptors.Frontiers in molecular neuroscience · 2023Article
- Ectopic Odorant Receptor Responding to Flavor Compounds: Versatile Roles in Health and Disease.Pharmaceutics · 2021Review
- Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry.Biotechnology letters · 2021Article
- 2D Nanomaterial-Based Surface Plasmon Resonance Sensors for Biosensing Applications.Micromachines · 2020Review
- Initial Characterization of a Subpopulation of Inherent Oscillatory Mammalian Olfactory Receptor Neurons.Chemical senses · 2019Article
- Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase.Journal of visualized experiments : JoVE · 2019Article
- Vapor detection and discrimination with a panel of odorant receptors.Nature communications · 2018Article
- Peripheral Gene Therapeutic Rescue of an Olfactory Ciliopathy Restores Sensory Input, Axonal Pathfinding, and Odor-Guided Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein GScientific reports · 2017Article
- Activation of odorant receptor in colorectal cancer cells leads to inhibition of cell proliferation and apoptosis.PloS one · 2017Article
- Olfactory Receptors in Non-Chemosensory Organs: The Nervous System in Health and Disease.Frontiers in aging neuroscience · 2016Review
- Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo.Nature neuroscience · 2015Article
- Receptive range analysis of a mouse odorant receptor subfamily.Journal of neurochemistry · 2015Article
- CD36 is involved in oleic acid detection by the murine olfactory system.Frontiers in cellular neuroscience · 2015Article
- Characterization of the Olfactory Receptors Expressed in Human Spermatozoa.Frontiers in molecular biosciences · 2015Article
- Human olfactory receptor responses to odorants.Scientific data · 2015Article
- Identification of agonists for a group of human odorant receptors.Frontiers in pharmacology · 2015Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we provide the first evidence for functional expression of a human olfactory receptor protein (OR17-40) and show that recombinant olfactory receptors can be functionally expressed in heterologous systems. A mixture of 100 different odorants (Henkel 100) elicited a transient increase in intracellular [Ca(2+)] in human embryonic kidney 293 (HEK293) cells stably or transiently transfected with the plasmid pOR17-40. By subdividing the odorant mixture into progressively smaller groups, we identified a single component that represented the only effective substance: helional. Only the structurally closely related molecule heliotroplyacetone also activated the receptor. Other compounds, including piperonal, safrole, and vanillin, were completely ineffective. Mock-transfected cells and cells transfected with other receptors showed no change in intracellular [Ca(2+)] in response to odor stimulation. We were also able to functionally express OR17-40 in Xenopus laevis oocytes. Coexpression of a "reporter" channel allowed measurement of the response of oocytes injected with the cRNA of the human receptor to the odor mixture Henkel 100. The effective substances were the same (helional, heliotropylacetone) as those identified by functionally expressing the receptor in HEK293 cells and were active at the same, lower micromolar concentration. These findings open the possibility of now characterizing the sensitivity and specificity of many, if not all, of the hundreds of different human olfactory receptors.
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10460249PMC6782526W2122306526What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.